姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响

Effects of curcumin on related gene expression during HaCaT cell apoptosis

目的:研究姜黄素对人永生化表皮HaCaT细胞凋亡过程中相关基因表达的影响。方法:通过免疫细胞化学法和免疫印迹检测姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响。结果:免疫细胞化学染色结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达减弱,促凋亡蛋白P53、Fas、Bax表达增强。Western blot检测结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达下调,促凋亡蛋白P53、Fas、Bax表达上调。结论:姜黄素诱导HaCaT细胞凋亡过程中伴随着抑凋亡基因bcl-2表达的抑制和促凋亡基因p53、Bax和Fas基因的激活。由此证明姜黄素通过多种凋亡相关基因的调控,激活凋亡信号传导途径,诱使细胞凋亡。

To explore the effects of curcumin on apoptosis-related gene expression in HaCaT cells. METHODS：Immunocytochemistry and Western blot were used to detect the effects of curcumin on apoptosis-related protein expression. RESULTS：Immunocytochemistry staining results showed that the expression levels of P53,Bax,Fas were upregulated significatntly while the products of Bcl-2 proteins were downregulated significantly in cells treated with 7.5 μg/mL curcumin. Western blot analysis confirmed the same results. CONCLUSION：Curcumin induced apoptosis of HaCaT cells by inhibiting expression of Bcl-2 gene and promoting the apoptosis genes p53,Bax and Fas activation. Curcumin could induce apoptosis by regulation of a variety of apoptosis-related genes to activate the apoptosis signaling pathway.