甘肃汉族妇女XRCC1和CCNH基因多态性与乳腺癌及乳腺良性肿瘤易感性研究

The relevance of XRCC1 and CCNH gene polymorphism to breast cancer and benign breasttumor in Han women of Gansu province

目的:研究甘肃地区汉族妇女XRCC1 rs25487、CCNH rs2234942基因多态性与乳腺癌及乳腺良性肿瘤发病的相关性。方法:选取经病理组织学确诊的乳腺癌、乳腺良性肿瘤各101例,匹配相同数量健康人作对照。使用聚合酶链式反应-限制性片段长度多态分析技术(PCR-RFLP)对XRCC1、CCNH进行基因型分析,通过Logistic回归分析不同基因型和临床病理特征与乳腺癌发病2的风险性关系,通过检验比较两种基因位点不同基因型的初潮年龄、发病年龄与乳腺癌和乳腺良性肿瘤的相关性。结果:Logistic回归分析发现XRCC1 rs25487位点GG基因型携带者的妇女罹患乳腺癌的危险性增加(P=0.001,OR=6.39,95%CI:2.18^+-18.65);临床病理免疫组化分析显示,XRCC1基因rs25487位点携带AA/AG基因型者,在PR与PR乳腺癌组织间的分布差异有显著+-2性(P=0.04,OR=0.29);携带AG/GG基因型者,在Her-2与Her-2乳腺癌组织间的分布差异有显著性(P=0.008,OR=0.45)。检验显示,乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487位点GG/AG基因型携带者的初潮年龄差异有显著性(P=0.001、0.043);乳腺癌和乳腺良性肿瘤患者CCNH rs2234942位点GG基因型携带者的初潮年龄差异有显著性(P=0.049);乳腺癌和乳腺良性肿瘤患者XRCC1rs25487和CCNH rs2234942位点GG基因型携带者,发病年龄差异有显著性(P=0.019、0.048)。结论:XRCC1 rs25487 GG基因型将会+增加乳腺癌的发病风险,XRCC1 rs25487位点携带AA/AG基因型者,在PR时乳腺癌的发病风险下降;携带AG/GG基因型者,在+Her-2时可能降低乳腺癌的发病风险。

To study the association between XRCC1 rs25487 and CCNH rs2234942 polymorphism with the susceptibility to breast cancer and benign breast tumor in Han women in Gansu area. METHODS：Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） assay was used for the polymorphism of XRCC1,CCNH in 101 cases of breast cancer,101 benign breast tumors and 101 disease-free controls collected in Gansu. Logistic regression analysis was used for comparing the genotypes or clinical pathological characteristics with the risk of breast cancer. Chi-square test was used to compare menarche age and disease onset age with the risk of breast cancer or benign tumor. RESULTS：Logistic regression analysis showed that XRCC1 rs25487,GG genotype increased the risk of breast cancer （P=0.001,OR=6.39,95%CI：2.18-18.65）. Regarding the clinicopathological immunohistochemical characteristics,the distribution of AA/AG genotype was significantly different＋ -between PR and PR at XRCC1 rs25487（P=0.04,OR=0.29）,while the distribution of AG/GG genotype was significantly＋ -different in Her-2 cases and Her-2 （P=0.008,OR=0.45）. Chi-square test found that the menarche age of GG/AG genotype in XRCC1 rs25487 and GG genotype in CCNH rs2234942 was significantly different in breast cancer and benign tumor （P=0.001,0.043,0.049）. The disease onset age of GG genotype in both XRCC1 rs25487 and CCNH rs2234942 was significantly different in breast cancer and benign tumor（P=0.019,0.048）. CONCLUSION：GG genotype in＋XRCC1 rs25487 increased the risk of breast cancer. AA/AG genotype in XRCC1 rs25487 with PR and AG/GG genotype in＋CCNH rs2234942 with Her-2 reduced the risk of breast cancer.