邻苯二甲酸二酯与特发性少弱精子症关系的探讨

Correlation between di-2-ethylhexyl phthalate and idiopathic oligoasthenospermia

目的:通过检测精浆中邻苯二甲酸二酯(DEHP)的含量,探讨DEHP与男性特发性少弱精子症之间的关系。方法:收集潍坊地区3家医院特发性少弱精子症所致不育患者100例,分为A组:常年接触蔬菜大棚塑料薄膜者50例;B组:常年食用塑料包装盒饭者50例;另设立C组(对照):为精液正常之志愿者50例。采用计算机辅助精子分析仪检测精子浓度和活率,采用反相液相高效色谱法测定精浆中DEHP的浓度。结果:1A、B、C3组中DEHP含量分别为(0.72±0.48)、(0.71±0.49)、(0.21±0.18)mg/L,C组与A、B组比较,差异均有统计学意义(P均<0.05)。2精浆中DEHP浓度与精子活率呈负相关(A组:r=-0.354,B组r=-0.348,P<0.05)。结论:常年接触某些塑料制品的男性不育患者,精浆中DEHP浓度高于正常人群,精浆内DEHP过量蓄积可能是不育的重要因素之一。

Objective： To investigate the relationship between the level of di-2-ethylhexyl phthalate （DEHP） and idiopathic oli- goasthenospermia by measuring the content of DEHP in the semen samples of different subjects. Methods ： We obtained semen sam- ples from 100 infertile men with idiopathic oligoasthenospermia, 50 working all the year round in the plastic greenhouse （ group A） and the other 50 constantly dining from plastic meal boxes （ group B）. We also enrolled 50 normal male volunteers as controls （ group C）. We conducted semen analyses using a computer-assisted sperm analyzer, measured the DEHP concentration by reversed-phase high- pressure liquid chromatography, and subjected the data to statistic processing by t-test and correlation analysis. Results： The mean concentrations of DEHP in the seminal plasma were （0.72 ±0.48）, （0.71 ±0.49） and （0.21 ±0.18） mg/L in groups A, B and C, respectively, significantly higher in A and B than in C （ both P 〈 0.05 ）. The DEHP concentration was negatively correlated with sperm motility （P 〈0. 05 ）. Conclusion： The DEHP level in the seminal plasma is higher in infertile men frequently exposed to plastic products than in normal males and excessive DEHP may he one of the important factors of idiopathic male infertility.