大肠癌组织中TGF-β1和HIF-1α和VEGF的表达及其临床意义

Expression of TGF-β1 and HIF-1α and VEGF in colorectal cancer tissue and their clinical significances

目的:通过检测转化生长因子β1(TGF-β1)、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在大肠癌及癌旁组织中的表达情况,探讨其临床意义和预后价值。方法:采用免疫组织化学法检测100例大肠癌及相应的癌旁组织中TGF-β1、HIF-1α和VEGF的表达,分析其表达与大肠癌临床病理特征及预后的关系。结果:1)TGF-β1、HIF-1α和VEGF在大肠癌组织中阳性率分别为63%、59%和63%;在癌旁组织中阳性率分别为11%、22%和13%,三者在大肠癌组织中的表达明显高于癌旁组织(P<0.05)。2)TGF-β1的表达与肿瘤的浸润深度明显相关(P<0.05);HIF-1α、VEGF的表达与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期明显相关(P<0.05)。3)TGF-β1、HIF-1α与VEGF在大肠癌组织中的表达呈正相关(P<0.05、P<0.01);生存单因素分析显示HIF-1α、VEGF均与患者的预后有关(P均<0.05)。结论:大肠癌组织中TGF-β1、HIF-1α、VEGF表达与肿瘤的生物学特征密切相关,可能是肿瘤侵袭转移的机制之一;联合检测TGF-β1、HIF-1α、VEGF对预测肿瘤侵袭转移及判断预后具有一定价值。

Objective：To examine the expression of transforming growth factorβ1 （TGF-β1）,hypoxia inducible factor-1α （HIF-1α）,vascular endothelial growth factor（VEGF） in colorectal cancer tissues and corresponding adjacent noncancerous tissues and to analyze the clinical and prognostic significances.Methods：Immunohistochemistry was used to detect the expression of TGF-β1,HIF-1α,VEGF in 100 cases of colorectal cancer specimens and corresponding adjacent noncancerous tissues.Analyzed the expression of TGF-β1,HIF-1α and VEGF and their relationship with clinicopathological characteristics and prognosis.The possibility of these three proteins as the predictors of prognosis of patients with colorectal cancer was also explored.Results：1）The positive ratio of TGF-β1,HIF-1α,VEGF in colorectal cancer tissues was 63％,59％,63％,respectively,but in adjacent colorectal tissues,the positive ratio was 11％,22％ and 13％,respectively.There were significant differences between cancer tissues and adjacent tissues（P＜0.05）.2）The expression of TGF-β1 was found to have positive relationship with invasion depth（P＜0.05）,while the expression of HIF-1α and VEGF was significantly correlated with histologic type,lymph node metastasis,distant metastasis and TNM stage in colorectal cancer （P＜ 0.05）.3）A positive correlation was observed between the expression of TGF-β1,HIF-1α and the expression of VEGF in colorectal cancer tissues （P＜ 0.05,P＜ 0.01,respectively）.The expression of HIF-1α and VEGF was significantly related to the prognosis of patients through survival analysis （P ＜ 0.05,respectively）.Conclusion：The expression of TGF-β1,HIF-1α and VEGF were closely related to the biological characteristics in colorectal cancer.Detection of TGF-β1,HIF-1α and VEGF may have certain value in predicting invasion metastasis and prognosis of colorectal cancer.