摘要
目的:通过检测转化生长因子β1(TGF-β1)、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在大肠癌及癌旁组织中的表达情况,探讨其临床意义和预后价值。方法:采用免疫组织化学法检测100例大肠癌及相应的癌旁组织中TGF-β1、HIF-1α和VEGF的表达,分析其表达与大肠癌临床病理特征及预后的关系。结果:1)TGF-β1、HIF-1α和VEGF在大肠癌组织中阳性率分别为63%、59%和63%;在癌旁组织中阳性率分别为11%、22%和13%,三者在大肠癌组织中的表达明显高于癌旁组织(P<0.05)。2)TGF-β1的表达与肿瘤的浸润深度明显相关(P<0.05);HIF-1α、VEGF的表达与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期明显相关(P<0.05)。3)TGF-β1、HIF-1α与VEGF在大肠癌组织中的表达呈正相关(P<0.05、P<0.01);生存单因素分析显示HIF-1α、VEGF均与患者的预后有关(P均<0.05)。结论:大肠癌组织中TGF-β1、HIF-1α、VEGF表达与肿瘤的生物学特征密切相关,可能是肿瘤侵袭转移的机制之一;联合检测TGF-β1、HIF-1α、VEGF对预测肿瘤侵袭转移及判断预后具有一定价值。
Objective:To examine the expression of transforming growth factorβ1 (TGF-β1),hypoxia inducible factor-1α (HIF-1α),vascular endothelial growth factor(VEGF) in colorectal cancer tissues and corresponding adjacent noncancerous tissues and to analyze the clinical and prognostic significances.Methods:Immunohistochemistry was used to detect the expression of TGF-β1,HIF-1α,VEGF in 100 cases of colorectal cancer specimens and corresponding adjacent noncancerous tissues.Analyzed the expression of TGF-β1,HIF-1α and VEGF and their relationship with clinicopathological characteristics and prognosis.The possibility of these three proteins as the predictors of prognosis of patients with colorectal cancer was also explored.Results:1)The positive ratio of TGF-β1,HIF-1α,VEGF in colorectal cancer tissues was 63%,59%,63%,respectively,but in adjacent colorectal tissues,the positive ratio was 11%,22% and 13%,respectively.There were significant differences between cancer tissues and adjacent tissues(P<0.05).2)The expression of TGF-β1 was found to have positive relationship with invasion depth(P<0.05),while the expression of HIF-1α and VEGF was significantly correlated with histologic type,lymph node metastasis,distant metastasis and TNM stage in colorectal cancer (P< 0.05).3)A positive correlation was observed between the expression of TGF-β1,HIF-1α and the expression of VEGF in colorectal cancer tissues (P< 0.05,P< 0.01,respectively).The expression of HIF-1α and VEGF was significantly related to the prognosis of patients through survival analysis (P < 0.05,respectively).Conclusion:The expression of TGF-β1,HIF-1α and VEGF were closely related to the biological characteristics in colorectal cancer.Detection of TGF-β1,HIF-1α and VEGF may have certain value in predicting invasion metastasis and prognosis of colorectal cancer.
出处
《中国现代普通外科进展》
CAS
2014年第7期505-510,共6页
Chinese Journal of Current Advances in General Surgery
基金
山东省优秀中青年科学家科研奖励基金(BS2011SW046)