Sombati癫痫细胞模型中高迁移率族蛋白1的表达变化

Expression of high-mobility group box-1 in Sombati＇s cell models

目的 研究Sombati癫痫细胞模型中高迁移率族蛋白1（HMGB1）的变化规律. 方法 新生SD乳鼠断头后迅速钝性分离出双侧海马,取海马神经元体外培养至第9天,将神经元分为模型组（无镁细胞外液处理）和对照组（正常细胞外液处理）,应用Wester blotting技术检测海马神经元在接受相应处理12、24、72 h后HMGB1蛋白的表达变化情况. 结果 Wester blotting显示,在神经元接受相应处理后12、24 h,模型组细胞所含HMGB1量均明显低于对照组,差异有统计学意义（t=2.783,P=0.012;t=2.509,P=0.021）; 72 h时模型组HMGB1表达量却明显高于对照组,差异具有统计学意义（t=4.112,P=0.006）.模型组HMGB1蛋白表达量于造模后12h、24 h、72 h呈现逐渐增高趋势. 结论 Sombati癫痫细胞模型中HMGB1的表达呈时间依赖性,随时间延长表达增加,可能在癫痫的病理生理中起重要作用.

Objective To investigate the expression changes of high-mobility group box-1 （HMGB1） in Sombati＇s cell models.Methods Hippocampal neurons were collected from bilateral hippocampus in SD suckling mice; they were divided into experimental group （cultured with Mg2＋-free extracellular fluid） and control group （cultured in normal Mg2＋ levels extracellular fluid）.The protein expressions of HMGB1 in the neurons of these two groups were determined at the time points of 12,24and 72 h after treatmnent by Western blotting.Results Western blotting indicated that the expression of HMGB1 in the experimental group was significantly lower than that in the control group at the time points of 12,24 h after treatment （t=2.783,P=0.012; t=2.509,P=0.021）; but at the time point of 72 h,that in the experimental group was significantly higher （t=4.112,P=0.006）.In addition,from time points of 12 h （0.248±0.118）,24 h （0.333±0.216） to the time point of 72 h （0.857±0.253）,the expression of HMGB 1 showed upward trend.Conclusion The expressions of HMGB1 show time dependence in the epilepsy of Sombati＇s cell models,and HMGB 1 may plays an important role in the pathophysiology of epilepsy.