不同糖浓度对新生鼠海马神经元GIuR2和PTEN表达的影响

Dextrose effect on the GIuR2 and PTEN expression of hippocampus neurons in newborn rat

目的：观察不同糖浓度对新生大鼠海马神经元细胞谷氨酸代谢型受体GluR2和PTEN表达的影响.方法：建立原代新生大鼠海马神经元细胞培养方法,培养至第10天时,给予不同浓度右旋葡萄糖刺激.通过RT-PCR检测GluR2、PTEN mRNA表达变化,通过免疫细胞化学检测海马神经元细胞GluR2、巢蛋白表达变化.结果：新生大鼠海马神经元细胞培养至第10天时,在不同糖浓度刺激后,GluR2 mRNA表达明显下调;在糖刺激下PTEN mRNA表达先上调,随着糖浓度升高,表达呈现下降趋势.免疫细胞显色显示海马神经元细胞GluR2蛋白表达下调,巢蛋白表达也呈现下调趋势,PTEN蛋白表达与mRNA表达趋势相同.结论：糖浓度升高引起海马神经元细胞GluR2和巢蛋白表达下调,以及PTEN表达上调,与高糖对海马神经元的变性、凋亡等损伤性变化,可能是糖尿病脑病的发生机制之一.

Objective：To investigate the effect of dextrose on the expression of glutamate receptor GluR2 and PTEN for primary hippocampus neurons in neonatal rat.Methods：The rat hippocampus neurons was cultured primarily for 10 days and then were stimulated with different concentrations dextrose.The GluR2 and PTEN mRNA expressions were examined by RTPCR and the GluR2 and nestin protein expression was detected by immunohistochemistry in primary hippocampus neurons.Results：The GluR2 mRNA expression was significantly decreased in the hippocampus neurons stimulated with different concentrations dextrose.The PTEN mRNA expression was significantly increased compared to the control group.And the GluR2 and nestin protein expressions were significantly increased compared to the control group.The PTEN protein expression had the same tendency as the mRNA expression in the hippocampus neurons.Conclusion：The decreased GluR2 and nestin expression and the increased PTEN expression in the neonatal primary hippocampus neurons may be injured for neuronal metabolic effects under the high stimulation of dextrose,indicating that the damage mechanism may be related to the incidence of diabetic encephalopathy.