肺炎支原体快速培养法在呼吸道感染诊断中的应用

Clinical application of mycoplasma pneumoniae rapid liquid culture in respiratory disease

目的：探讨快速培养法在诊断肺炎支原体导致的呼吸道感染中的作用。方法对2011年12月～2013年12月来院就诊的疑似肺炎支原体感染患者200例进行研究，留取咽拭子和血清，分别进行快速培养法、荧光定量PCR法和血清抗体法检验，并就快速培养法与其余两种不同的检测方法的结果进行对比分析。结果快速培养法、荧光定量PCR法和血清抗体检验法检测阳性率分别为30.0%（60/200）、29.0%（58/200）和16.5%（33/200），快速培养法阳性率与荧光定量PCR法检测阳性率相比较，差异无统计学意义（P＞0.05）；而快速培养法检测阳性率与血清抗体法检测阳性率相比较，快速培养法检测阳性率显著高于血清抗体检测法，且差异具有统计学意义（P＜0.05）。结论快速培养法检测肺炎支原体的敏感性和灵敏性可与荧光定量PCR法检测结果相媲美，但装备技术要求和检测成本明显低于荧光定量PCR法，同时敏感性和灵敏性显著高于血清抗体检测法。快速培养法检测肺炎支原体不仅能有效满足临床需要，同时兼具有成本低的特点，因此这种方法具有较高的性价比，尤其适用于基层医院。

Objective To investigate the early diagnosis of pulmonary mycoplasma pneumoniae infection. Methods 200 cases adults with respiratory infection was tested by sputum, pharynx swab culture and fluorogenic quantitative PCR. The diagnosis results were compared in the same cases. Results The positive rate of the pharynx swab culture and fluorogenic quantitative PCR was 30.0%（60/200）,29.0%（58/200）respectively. There was no significant difference between the two methods（P 〉 0.05）. However,the positive rate of the sputum was lowest in the three methods. There was a significant different in the comparison of the data（P〈0.05）. Conclusion It would get a satisfactory diagnosis effect to use mycoplasma pneumoniae rapid liquid culture in respiratory disease.It has the same effect with the PCR, but lower cost and the need of equipment requirement.