Bio-Gide胶原膜对兔骨髓间充质干细胞增殖和成骨分化的影响

Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells

背景:相关实验表明Bio-Gide胶原膜与细胞有良好的生物相容性,但有关与其复合培养干细胞成骨分化能力的报道少见。目的:观察Bio-Gide胶原膜对骨髓间充质干细胞增殖及成骨分化的影响。方法:全骨髓贴壁法体外分离培养兔骨髓间充质干细胞,将第3代兔骨髓间充质干细胞分别接种于覆盖Bio-Gide胶原膜的培养板(实验组)与单纯培养板(对照组)培养。于培养1,4,7,14 d利用CCK-8试剂盒检测细胞增殖;成骨分化诱导培养1,4,7,14 d收集细胞培养液上清,检测细胞碱性磷酸酶活性。结果与结论:两组细胞数量均随着培养时间的增加而不断增加,对照组培养7 d细胞数量明显多于实验组(P<0.05),其他时间点组间比较差异无显著性意义。两组细胞碱性磷酸酶活性均随着培养时间的增加而不断增加,实验组成骨诱导14 d细胞碱性磷酸酶活性高于对照组(P<0.05),其他时间点组间比较差异无显著性意义。表明Bio-Gide胶原膜可促进兔骨髓间充质干细胞的增殖及成骨分化。

BACKGROUND：Bio-Gide col agen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide col agen membranes is rarely reported. OBJECTIVE：To observe the effect of Bio-Gide col agen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells. METHODS：Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide col agen membrane pretreated petri dish （experimental group） and simple petri dish （control group）. The proliferation of bone marrow mesenchymal stem cells was detected by cellCounting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were col ected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days. RESULTS AND CONCLUSION：The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days （P〈0.05）. There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days （P〈0.05）. There was no significant difference between the two groups＆amp;nbsp;at other time points. Experimental findings indicate that Bio-Gide col agen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.