β-连环蛋白和肝细胞核因子1α在肝细胞癌组织中的表达及其对预后的影响

Expression of β-catenin and HNF-lα and their influence on prognosis in human hepatocellular carcinoma

目的探讨β-连环蛋白（β-catenin）和肝细胞核因子1α（HNF-1α）在肝细胞癌（HCC）组织中的表达及其对预后的影响。方法采用免疫组化方法检测50例HCC组织、50例癌旁肝硬化组织和7例肝血管瘤旁肝组织中β-catenin和HNF-1α的表达情况。结果β-catenin在HCC组织、癌旁肝硬化组织和肝血管瘤旁肝组织中的异常表达率分别为74．0％（37／50）、18．O％（9／50）和14．3％（1／7），β-catenin在HCC组织中的异常表达率高于肝血管瘤旁肝组织（P=0．002）和癌旁肝硬化组织（P〈0．001），且β-catenin异常表达患者的预后差。在50例HCC组织中，HNT-1α阴性10例（20．0％），HNF-lα弱阳性20例（40．O％），HNF-lα中度阳性13例（26．0％），HNF．10t强阳性7例（14．0％）。在50例癌旁肝硬化组织中，HNF-1α阴性6例（12．0％），HNF-1α弱阳性10例（20．0％），HNF-1α中度阳性26例（52．0％），HNF-1α强阳性8例（16．O％）。在7例肝血管瘤旁肝组织中，HNF-lα弱阳性1例（14．3％），HNF-lα中度阳性2例（28．6％），HNF-lα强阳性4例（57．1％）。HNF-lα在HCC组织中的表达显著低于癌旁肝硬化组织（P=0．008）和肝血管瘤旁肝组织（P=0．029），HNF-lα阳性表达患者的预后较好。β-catenin异常表达与HNF-lα表达强度呈负相关（r=-0．673，P〈0．001）。结论HCC的发生、发展与β-eatenin的异常表达有关。在HCC中，Wnt／13-catenin信号通路与HNF-1α仪的表达有一定的关系。

Objective To study the expressions of β-catenin in hepatocellular carcinoma （HCC） tissue, adjacent cirrhotic liver tissue and hemangioma-surrounding liver tissue to understand whether their difference in expression will influence on the prognosis and to study the relationship between Wnt/β-catenin signaling pathway and HNF-lαexpression. Methods 50 specimens of HCC, 50 specimens of adjacent cirrhotic liver tissue and 7 specimens of hemangioma-surrounding liver tissue were used to detect the differences in the expression of β-catenin and HNF-lα in them by immunohistochemistry. Results The expression rate of β-catenin was 74.0% （37/50） in the HCC tissue, 18.0% （9/50）in cirrhotic liver tissue, and 14.3% （1/7） in hemangioma-surrounding liver tissue. The expression rate of β-catenin in HCC tissue was significantly higher than that in the hemangioma-surrounding liver tissue （ P = O. 002 ） and cirrhotic liver tissue （P 〈 0. 001 ）. The patients with abnormal expression had worse prognosis. Among the 50 HCC cases, the expression of HNF-lα was negative in 20.0% （ 10/50）, weak positive in 40.0% （20/50）, moderately positive in 26.0% （13/50）, and strong positive in 14.0% （7/50）. Among the 50 adjacent cirrhotic liver tissues, the expression of HNF-1α was negative in 12.0% （6/50）, weak positive in 20.0% （10/50）, moderately positive in 52.0% （26/50） and strong positive in 16.0% （8/50）. In the 7 eases of hemangioma-surrounding liver tissue, the expression of HNF-lα was negative in 0 （0/7） , weak positive in 14.3% （ 1/7）, moderately positive in 28.6% （2/7） and strong positive in 57.1% （4/7）. The positive expression rate of HNF-lα in the HCC tissue was significantly lower than that in the hemangioma- surrounding liver tissues（P = 0. 029 ） and adjacent cirrhotic liver tissues （ P = 0. 008 ）. The patients with positive HNF-lα expression had a better prognosis. The abnormal expression of β-eatenin was negatively correlated with positive HNF-1α expression （r = - 0.673, P 〈 O. 001 ）. Conelttsions The occurrence and development of HCC is related to the abnormal β-catenin expression. There is a negative correlation between Wnt/β-catenin signaling pathway and HNF-1α expression.