摘要
目的探讨β-连环蛋白(β-catenin)和肝细胞核因子1α(HNF-1α)在肝细胞癌(HCC)组织中的表达及其对预后的影响。方法采用免疫组化方法检测50例HCC组织、50例癌旁肝硬化组织和7例肝血管瘤旁肝组织中β-catenin和HNF-1α的表达情况。结果β-catenin在HCC组织、癌旁肝硬化组织和肝血管瘤旁肝组织中的异常表达率分别为74.0%(37/50)、18.O%(9/50)和14.3%(1/7),β-catenin在HCC组织中的异常表达率高于肝血管瘤旁肝组织(P=0.002)和癌旁肝硬化组织(P〈0.001),且β-catenin异常表达患者的预后差。在50例HCC组织中,HNT-1α阴性10例(20.0%),HNF-lα弱阳性20例(40.O%),HNF-lα中度阳性13例(26.0%),HNF.10t强阳性7例(14.0%)。在50例癌旁肝硬化组织中,HNF-1α阴性6例(12.0%),HNF-1α弱阳性10例(20.0%),HNF-1α中度阳性26例(52.0%),HNF-1α强阳性8例(16.O%)。在7例肝血管瘤旁肝组织中,HNF-lα弱阳性1例(14.3%),HNF-lα中度阳性2例(28.6%),HNF-lα强阳性4例(57.1%)。HNF-lα在HCC组织中的表达显著低于癌旁肝硬化组织(P=0.008)和肝血管瘤旁肝组织(P=0.029),HNF-lα阳性表达患者的预后较好。β-catenin异常表达与HNF-lα表达强度呈负相关(r=-0.673,P〈0.001)。结论HCC的发生、发展与β-eatenin的异常表达有关。在HCC中,Wnt/13-catenin信号通路与HNF-1α仪的表达有一定的关系。
Objective To study the expressions of β-catenin in hepatocellular carcinoma (HCC) tissue, adjacent cirrhotic liver tissue and hemangioma-surrounding liver tissue to understand whether their difference in expression will influence on the prognosis and to study the relationship between Wnt/β-catenin signaling pathway and HNF-lαexpression. Methods 50 specimens of HCC, 50 specimens of adjacent cirrhotic liver tissue and 7 specimens of hemangioma-surrounding liver tissue were used to detect the differences in the expression of β-catenin and HNF-lα in them by immunohistochemistry. Results The expression rate of β-catenin was 74.0% (37/50) in the HCC tissue, 18.0% (9/50)in cirrhotic liver tissue, and 14.3% (1/7) in hemangioma-surrounding liver tissue. The expression rate of β-catenin in HCC tissue was significantly higher than that in the hemangioma-surrounding liver tissue ( P = O. 002 ) and cirrhotic liver tissue (P 〈 0. 001 ). The patients with abnormal expression had worse prognosis. Among the 50 HCC cases, the expression of HNF-lα was negative in 20.0% ( 10/50), weak positive in 40.0% (20/50), moderately positive in 26.0% (13/50), and strong positive in 14.0% (7/50). Among the 50 adjacent cirrhotic liver tissues, the expression of HNF-1α was negative in 12.0% (6/50), weak positive in 20.0% (10/50), moderately positive in 52.0% (26/50) and strong positive in 16.0% (8/50). In the 7 eases of hemangioma-surrounding liver tissue, the expression of HNF-lα was negative in 0 (0/7) , weak positive in 14.3% ( 1/7), moderately positive in 28.6% (2/7) and strong positive in 57.1% (4/7). The positive expression rate of HNF-lα in the HCC tissue was significantly lower than that in the hemangioma- surrounding liver tissues(P = 0. 029 ) and adjacent cirrhotic liver tissues ( P = 0. 008 ). The patients with positive HNF-lα expression had a better prognosis. The abnormal expression of β-eatenin was negatively correlated with positive HNF-1α expression (r = - 0.673, P 〈 O. 001 ). Conelttsions The occurrence and development of HCC is related to the abnormal β-catenin expression. There is a negative correlation between Wnt/β-catenin signaling pathway and HNF-1α expression.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2014年第8期587-591,共5页
Chinese Journal of Oncology
基金
国家自然科学基金(81072966)
河北省自然科学基金(C2007001063)
关键词
癌
肝细胞
肝硬化
肝血管瘤
Β-连环蛋白
肝细胞核因子lα
预后
Carcinoma, hepatocellular
Liver cirrhosis
Hemangioma
13-catenin
Hepatocyte nuclear factor -lot
Prognosis