坤泰胶囊对体外受精卵巢低反应患者获卵数、卵细胞及胚胎质量的影响

Effect of Kuntai Capsule on the Number of Retrieved Oocytes,High-quality Oocytes and Embryos in in Vitro Fertilization of Poor Ovarian Response Patients

目的观察坤泰胶囊对体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)卵巢低反应(poor ovarian response,POR)患者获卵数、卵细胞和胚胎质量的影响。方法 70例将行IVF-ET的POR患者随机分为观察组和对照组,每组35例。观察组在IVF-ET超排卵周期的前3个月经周期(简称准备周期)以及超排卵过程中联合应用坤泰胶囊,对照组在此过程中联合应用安慰剂。观察两组用药前后肾阴虚证候改善情况;基础促卵泡刺激素(basaI follicIe-stimuIating hormone,bFSH)、促黄体生成激素(luteinizing hormone,LH)、雌激素(estradioI,E_2)、抗苗勒氏管激素(anti-M(u|¨)llerian hormone,AMH)、FSH/LH、窦卵泡数(antraI follicIe count,AFC)情况;两组HCG日单个卵E_2水平、获卵数、优质卵率、优质胚胎率。结果观察组肾阴虚证候改善情况、bFSH、LH水平下降、AFC增加数目、HCG日单个卵E_2水平、获卵数、优质卵率、优质胚胎率均优于对照组,差异有统计学意义(P<O.05);FSH/LH水平下降两组比较差异无统计学意义(P>0.05);E_2、AMH水平观察组服药后比服药前升高,对照组服药后比服药前下降,两组E_2、AMH差值比较,差异有统计学意义(P<0.05)。结论坤泰胶囊可增加IVF-ET中POR患者的获卵数,提高卵细胞和胚胎质量。

Objective To observe the effect of Kuntai Capsule （KC） on the number of retrieved oocytes, the quality of high-quality oocytes and embryos in in vitro fertilization of poor ovarian response （POR） patients. Methods Totally 70 POR patients preparing for in vitro fertilization-embryo transfer （IVF-ET） were randomly assigned to the observation group and the control group, 35 cases in each group. KC was administered to patients in the observation group in the preparation cycle （i.e., three men- strual cycles before IVF-ET） and during the superovulation process. Those in the control group took placebo during this period. Before and after medication the improvement of Shen yin deficiency syndrome （SYDS） was observed in the two groups. The basal follicle-stimulating hormone （bFSH）, luteinizing hor- mone （LH）, estradiol （E2）, anti-Mtillerian hormone （AMH）, the ratio of FSH to LH, and antral follicle count （AFC） were observed. Besides, the E2 level of a single ovum on the day of HCG injection, the number of retrieved oocytes, the high-quality oocyte rate, and the high-quality embryos were observed. Results Compared with the control group, the SYDS, decreased bFSH and LH levels, increased ACF numbers, the E2 level of a single ovum on the day of HCG injection, the number of retrieved oocytes, high-quality oocytes, and high-quality embryos were superior in the observation group （P 〈0.05）. There was no statistical difference in the decreased FSH/LH level （P 〉0.05）. E2 and AMH increased after medi- cation of KC in the observation group, while they decreased after administration of placebos in the control group. There was statistical difference in the post-pre treatment difference of E_2 and AMH between the two groups （P 〈 0.05）. Conclusion KC could increase the number of retrieved oocytes, and elevate the quality of occytes and embryos in the IVF-ET.