Parkin基因在鼻咽癌中转录表达及临床意义

mRNA expression of Parkin gene in human nasopharyngeal carcinoma and its clinical significance

目的研究Parkin基因在鼻咽癌中转录表达及临床意义。方法运用半定量逆转录PCR的方法检测54例鼻咽癌组织和16例正常鼻咽上皮组织Parkin基因转录表达水平,分析Parkin基因转录表达与鼻咽癌患者临床病理特征关系。结果 54例鼻咽癌组织Parkin基因相对转录表达水平为0.3430±0.4947,16例正常鼻咽上皮组织Parkin基因相对转录表达水平为1.0052±0.4911,鼻咽癌组织Parkin转录表达明显下调,Parkin基因转录表达与患者年龄、性别、T分级、TNM分期、病理类型无明显关系,而与淋巴结转移有关。结论 Parkin在鼻咽癌的发生发展中起抑癌基因作用,Parkin基因转录表达可作为判断鼻咽癌临床预后预测指标。

OBJECTIVE To study the mRNA expression of Parkin gene in human nasopharyngeal carcinoma （NPC） and its clinical significance. METHODS The semi-quantitative reverse transcription PCR （RTPCR） was used to detect the mRNA expression level of Parkin gene in 54 human NPC tissues and 16 normal nasopharyngeal epithelial tissues. The relationship between mRNA expression of the gene and clinical data was analyzed. RESULTS The mRNA expression level was significantly decreased in 54 human NPC tissues （0.3430± 0.4947） than that in 16 normal nasopharyngeal epithelia tissues （1.0052±0.4911）. The decreased mRNA expression of Parkin was not related with age, gender, T stage, TNM classification and histopathologic subtype, but was related with N stage. CONCLUSION Parkin gene mRNA expression may play an important role in the genesis and development of human NPC. The mRNA expression of Parkin may serve as an important indicator in predicting the prognosis of human NPC.