SOCS3在大鼠急性胰腺炎早期炎症反应过程中对NF-κB的影响

Relationship between SOCS3 and NF-κB during the early inflammatory response in acute pancreatitis

目的探讨SOCS3在急性胰腺炎(alute pancreatitis,AP)早期炎症反应过程中与NF-κB的关系,为AP早期炎症反应过程的治疗提供理论依据。方法 48只雄性Sprague-Dawley(SD)大鼠随机分为2组:假手术组(8只)、AP造模组(40只)。假手术组开腹后翻动肠管;AP造模组以4%牛磺胆酸钠胰胆管逆行注射诱导模型。各组分别于术后3 h、6 h、12 h、18 h、24 h抽血检测血清淀粉酶(AMY),并测定腹水量;光镜下观察胰腺病理形态学改变;采用免疫组化法和Western blotting法检测SOCS3和NF-κBp65在胰腺中定位和蛋白表达水平。结果与假手术组比较,AP各组胰腺病理损伤程度随病情进展逐渐加重,淀粉酶(AMY)水平均明显升高,腹水量明显增多(P<0.05);在AP病程进展的不同时段,SOCS3和NF-κBp65表达水平均明显高于假手术组,且各组间比较,差异有统计学意义(P<0.05)。结论 SOCS3和NF-κB均参与AP早期胰腺损伤及炎症反应过程,并与AP严重程度、持续时间密切相关,可能在胰腺局部及全身炎症反应的调节中起重要作用。

Objective To investigate the relationship between SOCS3 and NF-κB during the early inflammatory re- sponse in acute pancreatitis （AP）. Methods Forty-eight male SD rats were randomly divided into two groups： sham operation group 8 rats and AP group 40 rats. The rats of sham operation group were flipped the bowel after lapardtomy. The rat model of AP were reproduced by retrograde infusion of 4% sodium taurocholate into the biliopancreatic duct and then colleced samples after 3 hours, 6 hours, 12 hours, 18 hours and 24 hours later. The levels of serum amylase （AMY） and ascites were detected. The pathological changes of pancreas were observed under the light microscope. The expression of SOCS3 and NF-κBp65 in pancreas were determined by Western blotting and immunohistochemistry. Re- sults Compared with sham operation group, the microscopic pancreas were gradually aggravated with disease progres- sion. The serum level of AMY and ascites level were increased significantly （P 〈 0.05）. The SOCS3 and NF-κBp65 ex- pression were significantly higher in AP group than those in the sham operation group （P 〈 0.05）. Conclusion SOCS3 and NF-κB were positively correlated in early pancreatic injury and inflammatory reactions in AP, which may play an important role in the pancreas and systemic inflammatory.