阴囊热应激引起小鼠生殖系统HSP90α表达变化和抗氧化能力改变

Scrotal Heat Stress Causes an Alteration of Anti- oxidative Ability and Induction of HSP90α Expression in Heat- stressed Mice

目的探讨阴囊急性热应激后小鼠抗氧化酶活性的变化和生殖系统热休克蛋白90α(HSP90α)的表达变化及规律。方法将56只8周龄雄性小鼠随机分为7组:对照组和6个热应激处理组(热应激后再置于室温环境中,时间点为0、1、4、12、24、48h)。分别测定各组小鼠的相应脏器指数,检测精子相关指标、睾丸和附睾中HSP90α的表达、血清中抗氧化酶的活性。结果阴囊急性热应激处理后,小鼠睾丸和肝脏指数在48h内无显著变化,而附睾指数在0、1h时显著低于对照组;精子活力0h组显著低于对照组,精子畸形率12、24h时间点明显高于对照组,其他试验组与对照组相比差异无统计学意义;0、1、4、12h组睾丸中HSP90α的表达均显著高于对照组(P<0.05),0、1、4、12、24h组附睾中HSP90α的表达明显高于对照组(P<0.05),其他试验组与对照组无统计学差异(P>0.05);0、4h血清中超氧化物歧化酶(SOD)活性显著升高(P<0.05),24、48h组和对照组相比无统计学差异(P>0.05)。结论急性阴囊热应激可引起小鼠血清中SOD活性升高和生殖系统损伤及睾丸和附睾中HSP90α的高表达。HSP90α的表达随时间变化且在热应激后48h基本恢复正常,提示其可能保护机体受到高热损伤。

Objective To investigate the changes of anti-oxidative ability and Hsp90α gene expression in reproductive system of mice with acute heat stress in scrotal.Methods Healthy 56 male mice,8 weeks old,were randomly divided into 7 groups：control group and six heat stress treatment group （in room temperature at 0,1,4,12,24 and 48h after heat stress）.Organ index,antioxidant enzyme activities,and the expression HSP90α gene in testicle and epididymis were determined.Results Mouse testis and liver index had no significant change at 48h,but at 0h and 1h the epididymis index was significantly higher than that in the controlgroup.Sperm deformity rate was significantly higher than that in the control group at 12h and 24h.Sperm motility was significantly lower than that in the control group at 0h and there was no difference compared with the control group at other times（P ＞0.05）.Gene expression of HSP90α in testicle was significantly higher than that in the control group at 0,1,4 and 12h.Gene expression of HSP90α in epididymis at 0,1,4,12 and 24h was significantly higher than it in the control group （P ＜ 0.05）.At 0h and 4h,SOD level in serum increased significantly after scrota1 treated with heat stress （P ＜ 0.05）,but at 24h or 48h,there was no significant difference compared with the control group （P ＞ 0.05）.Conclusion Acute heat stress in scrotal resulted in the increased SOD level in serum and the damage of reproductive system,and the increased expression of HSP90α gene in testicle and epididymis.The increased expression of HSP90α gene was almost restored to the normal level at 48h after heat stress,suggesting it may protect cells from heat damage.