不同氧浓度对三维体外着床模型中内膜间质细胞的影响

Investigation on the Effect of Different Oxygen Tensions on Endometrial Stromal Cells via Three- dimensional in Vitro Embryo Implantation Model

目的本研究将基于已经建立的三维小鼠体外胚胎着床模型,将整个培养环境的氧浓度调整为2%,从而探究缺氧环境对胚胎着床的作用和影响。方法将已经建立的小鼠体外着床模型分为两组,其中一组置于氧浓度2%的密封箱内进行培养。另外一组为对照组,直接置于培养箱中,按照原先的大气氧浓度进行培养。观察对比这两组之间胚胎与内膜的黏着率。并对着床胚胎及内膜进行组织切片,观察组织的形态学变化,检测其中细胞凋亡的情况。结果小鼠囊胚在2%的氧浓度及大气氧浓度下的胚胎黏着率分别为41.4%及53.2%;这两组的胚胎黏着率比较,差异无统计学意义。两种情况下的小鼠胚胎均已经黏附于体外共培养的子宫内膜上皮层表面,未见明显形态学异常。但在2%氧浓度下,子宫内膜组织中很多间质细胞发生了细胞凋亡。结论 2%的氧浓度在三维体外胚胎着床模型的运用中,虽然没有导体外胚胎黏着率的显著降低,但却诱导了体外培养子宫内膜组织中细胞的凋亡。

Objective To study the effect of different oxygen tension on embryo attachment rate via a three-dimentional in vitro embryo implantation model,with the oxygen concentration decreasing to 2％ in the culture condition.Methods In vitro embryo implanration was performed on endometrium cultured under 2％ oxygen or atmospheric condition,and the effect on 3 parameters namely,the luminal epithelial thickness,apoptotic cell death and blastocysts attachment rate,were examined.Results There was no significantly different attachment rate between the 2％ oxygen group and the atmospheric condition group.There was no morphological change be found from the histological section of the two groups.However,there were much apoptotic stromal cells in the cultured endometrial tissue in 2％ oxygen.Conclusion 2％ oxygen induced apoptosis of the endometrium in culture,though it did not affect embryo attachment.