豚鼠低温死亡血清生化指标的分析

Analysis of Biochemical Markers in Serum of Guinea Pigs after Death Caused by Hypothermia

目的检测低温死亡豚鼠的血清生化指标的变化情况,并分析其变化规律,为利用生化指标推断低温死亡提供一定的参考。方法 20只豚鼠随机分为实验组和对照组,实验组置于-30℃左右环境下至死亡,对照组置于25℃左右环境下,经相应时间后再断颈处死。分别提取右心血血清,使用生化仪对一系列血清生化指标进行检测。结果相较于对照组,低温死亡豚鼠血清中葡萄糖、尿酸、肌酐及尿素氮含量升高(P<0.05),总蛋白和白蛋白含量降低(P<0.05),而其他指标如血清酶和血清离子等则无明显变化。结论低温死亡下的血清生化指标的变化存在一定的特征性,有望在低温死亡原因推断中起到辅助鉴定作用。

Objective To explore the changes and rules of biochemical markers in serum of guinea pigs after death caused by hypothermia and to provide references for fatal hypothermia diagnosis by serum biochemical markers. Methods Twenty guinea pigs were randomly divided into experimental group and control group. The guinea pigs in the experimental group were kept at -30 ℃until death, while the ones in control group were decapitated after same survival intervals at 25 ℃. The serum was extracted from the whole blood of right ventricular immediately. Subsequently, a series of serum biochemical markers were analyzed by auto bio-chemical analyzer. Results The levels of glucose, uric acid, creati- nine and urea nitrogen in the experimental group were significantly higher than those in control group, respectively （P〈0.05）. Compared with the control group, the levels of total protein and albumin were sig- nificantly lower in the experimental group （P〈0.05）. There were no significantly differences of the levels of other markers such as serum enzymes and ions observed between the two groups. Conclusion There are characteristic changes of some specific serum biochemical markers in fatal hypothermia, which may be potentially useful for auxiliary diagnosis of fatal hypothermia.