大鼠软组织挤压伤后NO对肝组织TNF-α和IL-1β基因表达的影响

Nitric Oxide Mediated TNF-α, IL-1β Gene Expression in Liver Induced by Crush Injury of Rat's Soft Tissues

目的探讨一氧化氮(nitric oxide,NO)在大鼠软组织挤压伤后肝组织TNF-α和IL-1β基因表达中的作用。方法将大鼠随机分成对照组、挤压组、挤压后氨基胍(aminoguanidine,AG)处理组、挤压后L-精氨酸(L-arginine,L-Arg)处理组,经实验处理后检测血清中ALT、AST活性和NO浓度,并利用RT-PCR技术观察肝组织中TNF-α、IL-1β基因表达情况。结果与对照组比较,挤压组肝组织中TNF-α、IL-1βmRNA表达明显上调(P<0.05),应用L-Arg后TNF-α、IL-1βmRNA表达进一步上调(P<0.05),而应用AG后上述指标明显下降(P<0.05);与对照组比较,挤压组血清ALT、AST活性以及NO浓度明显增加(P<0.05),应用L-Arg后血清ALT、AST活性以及NO浓度进一步明显增高(P<0.05),应用AG后上述指标则明显降低(P<0.05)。结论大鼠软组织挤压伤诱导NO生成增多,内源性NO介导肝组织TNF-α和IL-1β基因表达上调。

Objective To explore the effect of nitric oxide （NO） on the gene expression of hepaticTNF-et and IL-I by crush injury of rat＇s soft tissues. Methods Rats were randomly divided into shamgroup, crush group, crush＋aminoguanidine （AG） group, and crush＋L-arginine （L-Arg） group. Activitiesof ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-a and IL-1βwere detected with RT-PCR. Results Obvious increase in TNF-ot and IL-1β mRNA expression wasdetected in the crush group compared with the sham group （P〈0.05）. After pretreated L-Arg, expressionsof TNF-et and IL-1β mRNA were markedly increased （P〈0.05）. After pretreated AG, those indices ob-viously decreased （P〈0.05）. Activities of ALT and AST enhanced and NO level increased in the crushgroup compared with the sham group （P〈0.05）. Pretreatment with L-Arg or AG led to substantial in-creased or reduced activities of ALT and AST as well as NO levels, respectively. Conclusion Endoge-nous NO mediated TNF-a, IL-1β mRNA up expression in liver induced by increased production of NOafter crush injury of rat＇s soft tissues.