光谱法研究芦丁及槲皮素与牛血清白蛋白的作用

Interaction of Luteolin and Quercetin with Bovine Serum Albumin by Spectroscopic Methods

目的:比较芦丁和槲皮素与牛血清白蛋白(BSA)作用的差异,探讨黄酮类化合物C环3位羟基糖苷化与否对结合白蛋白的影响。方法:荧光光谱、紫外-可见吸收光谱、同步荧光光谱和圆二色谱法测定芦丁和槲皮素与BSA的作用。结果:在实验条件下,槲皮素对BSA的荧光猝灭作用明显强于芦丁。二者与BSA的作用以静态结合为主。低浓度的槲皮素影响BSA的酪氨酸的微环境,基本不影响BSA色氨酸的微环境;低浓度的芦丁对BSA的酪氨酸及色氨酸微环境基本不影响。二者在高浓度下都能够轻微改变BSA的二级构象。结论:黄酮C环3位羟基糖苷化会降低其对BSA的亲和力,影响与BSA之间的结合位点。

Objective：To compare the similarities and differences in interaction of Luteolin and Quercetin with bovine ser -um albumin（BSA）.Method： Fluorescence spectra, UV -Vis absorption spectroscopy ,synchronous fluorescence and cir-cular dichroism（CD） method were applied to investigate the interaction of Luteolin and Quercetin with BSA .Results：The fluorescence quenching effect of quercetin on BSA was stronger than that of Luteolin .Both Luteolin and Quercetin on BSA were mainly static combination .Quercetin in low concentrations could affect the tyrosine microenvironment , but could not affect the tryptophan microenvironment of BSA .Rutin in low concentrations could affect neither tyrosine nor tryptophan microenvironment of BSA .Both Luteolin and Quercetin at high concentration could slightly alter the second conformation of BSA.Conclusion：3 hydroxyl of flavonoid glycosylation will reduce its affinity for BSA and influence the binding sites with BSA.