生防菌XM-10抑菌活性测试及鉴定

Identification of Inhibition Activity of a Bio-control Strain XM-10

[目的]针对生防菌XM-10进行抑菌活性测试及鉴定,为该菌株开发成环境友好型生物制剂奠定基础。[方法]采用对峙培养法测试了其对小麦赤霉病、黄瓜枯萎病、苹果轮纹病、大豆菌核病等9种病原菌的抑菌活性,并根据其个体形态特征、培养性状以及16s rRNA序列分析进行了鉴定。[结果]结果表明XM-10菌株对供试的9种致病菌均有不同程度的抑制作用,抑菌率在47.8%~87.6%之间,其中对小麦赤霉病菌和黄瓜枯萎病菌的抑制率分别达到了87.6%和83.7%,抑菌带在4.9~6.9 mm之间;经形态观察和16s rRNA序列分析将该菌株初步鉴定为黄灰链霉菌。[结论]生防菌XM-10的抑菌谱较广,为放线菌中的链霉菌属,在病害生物防治中具有良好的应用潜力和前景。

A preliminary study on the antibacterial activity and antibacterial mechanism for the antagonistic effect on Fusarium oxysporurn wilt bio-control agent XM-10 was tested on 9 pathogens, such as Fusarium graminearum, Physalospora piricola, Sclerotinia sclerotiorura, etc. It was identified according to the morphology, cultural characters, physiological and biochemical characteristics and 16S rRNA sequence analysis. The results showed that the inhibitory effect of XM-IO strains to 9 species of pathogenic bacteria was between 47.8% and 87.6%, in which the inhaibitory effect to Fusarium graminearurn, Fusarium oxysporum were 87.6 and 83.7, respectively. The antibacterial belt was between 4.9 mm and 6.9mm. It was identified as yellow grey streptomycete according to morphology and 16S rRNA sequence analysis. It was concluded that bio-control strain XM-10 was streptomycete which had wide antibacterial effect. It was of great potential in bio-control of many diseases.