血必净对腹主动脉阻断合并脓毒症大鼠心肌的影响

Effect of Xuebijing injection on cardiac muscle in the rats undergoing abdominal aorta clamping with sepsis

目的观察血必净注射液对腹主动脉阻断术合并脓毒症大鼠心肌的保护作用。方法将Wistar大鼠32只随机分为对照组(S组)、腹主动脉阻断合并脓毒症组(I/R+LPS组)、血必净组(XBJ组)。XBJ组分离腹主动脉前30 min进行血必净预处理,尾静脉注射8 mL/kg血必净注射液,腹主动脉阻断再灌注2 h后,腹腔注射LPS 20 mg/kg。所有动物于腹主动脉阻断后8 h处死,取心脏组织行HE染色观察病理损害情况,免疫组化检测各组心脏TNF-α、β2肾上腺素能受体的表达情况。结果 1心脏组织病理学显示:S组未见明显的病理改变;I/R+LPS组心肌细胞明显肿胀,肌间隙增宽,小血管充血明显,内有大量红细胞聚集,肌间隙内可见大量红细胞漏出,纤维变性、坏死,肌间隙炎性细胞明显增多聚集、贴壁,甚至出现结构模糊的溶断现象;XBJ组心肌组织紊乱,心肌细胞略肿胀,肌间隙内可见红细胞漏出、炎性细胞浸润。2免疫组织化学染色检测结果:心肌TNF-α表达数量S组<XBJ组<I/R+LPS组,各组之间均有显著性差异(P均<0.05);心肌β2-AR表达数量S组>XBJ组>I/R+LPS组,XBJ组与I/R+LPS及S组间比较均无显著性差异,S组与I/R+LPS组间比较有显著性差异(P<0.05)。结论血必净注射液预处理对腹主动脉阻断合并脓毒症大鼠早期的心肌损伤起一定的保护作用;其在缺血再灌注合并脓毒症大鼠心肌损伤的早期模型中具有下调TNF-α的作用,而对心肌β2-AR无明显影响。

Objective It is to observe the protecting effect of Xuebijing injection（ XBJ） on cardiac muscle in the rats undergoing abdominal aorta clamping complicating with sepsis. Methods 32 Wistar rats were randomly divided into control group（ S group）,abdominal aorta clamping complicating with sepsis group（ I/R ＋ LPS group）,Xuebijing injection group（ XBJ group）. The rats in XBJ group were pretreated with XBJ 30 min before abdominal aorta separated,8 mL /kg by caudal vein injection,in 2 h after abdominal aorta ischemia reperfusion injury,they were given LPS 20 mg /kg by intraperitoneal injection.All the animals were killed in 8 h after abdominal aorta blocking,and their cardiac tissue was gotten to do HE staining to observe pathological damage,the expression of TNF- α and β2adrenoreceptor（ AR） in the tissue was detected by immunohistochemistry technique. Results 1Cardiac tissue pathology results showed that： no obvious pathological change was found in S group; In I /R ＋ LPS group myocardial cells were swelling with wider intermuscular space,little blood vessel was obviously hyperemia with agglutination of many red blood cells which were also leaked much in the space,myocardial fibers were degeneration and necrosis,many inflammatory cells gathered in the space,even the structure was indistinct in cardiac tissue; In XBJ group,myocardial tissue was disorder in which the cells were slight swelling,and less leak of red blood cells and infiltration of inflammatory cells in the space was found. 2Immunohistochemistry detection results showed that the expression of TNF- ɑ in cardiac cells： S group〈 XBJ group 〈I /R ＋ LPS group,the difference was significant among every group（ P〈0. 05）,the expression of β2- AR in the cells： S group 〉XBJ group 〉I /R ＋ LPS group,the difference between S group and I /R ＋ LPS group was significant but not in XBJ group compared with the other two groups. Conclusion XBJ pretreatment has some protecting effect on early myocardial injury in the rats undergoing abdominal aorta clamping complicating with sepsis,it can decrease the expression of TNF- α with no obvious effect on β2- AR in myocardial tissue in the rats.