舒郁胶囊对血管性抑郁模型大鼠行为学及脑组织单胺类神经递质的影响

Effects of “Shuyu Capsule” on behavior and cerebral cortex monoamine neurotransmitters in vascular depressive model rats

目的观察舒郁胶囊对血管性抑郁模型大鼠行为学及脑组织单胺类神经递质的影响。方法采用高脂饲养结合慢性轻度不可预见性应激刺激建立大鼠血管性抑郁模型。将实验大鼠随机分为正常组、模型组、舒郁胶囊组、氟西汀组,并给予相应的干预措施。观察大鼠干预前后行为学评分及检测大鼠脑内5-HT、NE、DA含量变化。结果与正常组比较,模型组、舒郁胶囊组、氟西汀组大鼠的水平运动和垂直运动显著减少、糖水偏嗜度明显下降(P<0.01),脑组织内5-HT、NE、DA含量显著减少(P<0.01)。与模型组比较,舒郁胶囊组和氟西汀组大鼠的水平运动和垂直运动显著增加、糖水偏嗜度明显升高(P<0.05,P<0.01),脑组织内5-HT、NE、DA含量显著增加(P<0.05,P<0.01);但两组之间各项目无显著性差异(P>0.05)。结论舒郁胶囊具有抗血管性抑郁作用,其作用机制与增加脑组织内神经递质含量有关。

Objective To observe the effects of＂Shuyu Capsule＂on behavior and cerebral cortex monoamine neurotransmitters in vascular depressive model rats.Methods The vascular depression animal models were established by high-fat feeding,and randomly divided into normal group,model group,＂Shuyu Capsule＂group,Fluoxetine group. Each group was treated by the corresponding measures. The behavioral score was observed and changes of 5-HT,NE and DA were detected. Results Compared with normal group,the horizontal movement,vertical movement and sucrose preference were remarkably decreased in the model group,＂Shuyu Capsule＂group and Fluoxetine group（P〈0. 01）,and the contents of 5-HT,NE and DA in brain tissues were remarkably decreased in the other three groups（P〈0. 01）. Compared with the model group,the horizontal movement,vertical movement,sucrose preference and contents of 5-HT,NE and DA in＂Shuyu Capsule＂group and Fluoxetine group were increased（P〈0.05,P〈0. 01）; no significant difference was found between the two groups（P〈0. 05）.Conclusion ＂Shuyu Capsule＂has anti-vascular depression effect. Its mechanism may be association with the regulation of monoamine neurotransmitters in brain.