骨疏颗粒对去卵巢雌性大鼠骨质疏松的影响

Effects of Gushu Granules on Ovariectomized Osteoporotic Model Rats Serum

目的观察骨疏颗粒对去卵巢骨质疏松模型大鼠雌二醇(estradiol,E2)、睾酮(testosterone,T)、孕酮(P)、泌乳素(prolacti,PRL)、卵泡刺激素(follicle-stimulating hormone,FSH)、促黄体生成素(luteinizing hormone,LH)的影响,为骨疏颗粒治疗骨质疏松症提供实验依据。方法 40只SD大鼠随机分为4组:模型组、正常对照组、雌激素组、骨疏颗粒组。采用卵巢摘除法制作骨质疏松模型,观察大鼠血清性激素相关6项检查E2、T、P、PRL、FSH、LH的变化、股骨骨质疏松病理及生化指标的变化。结果与模型组比较,骨疏颗粒可以改善去势雌性大鼠血清雌、雄激素水平,缓解骨质疏松病理变化,升高降钙素,降低骨碱性磷酸酶、肿瘤坏死因子、抗酒石酸碱性磷酸酶、尿羟脯氨酸(P<0.01或P<0.05)。结论骨疏颗粒能治疗和延缓骨质疏松,与雌激素比较差异无统计学意义。

Objective To investigate the effect of Gushu granules on Ovariectomized Osteoporotic Model Rats E2、T 、P 、PRL、FSH 、LH. In order to provide experimental basis for clinical application of Gushu granules and further study. Methods 40 SD rats were randomly divided into 4 groups：model group,blank control group,estrogen group and group of Gushu granules. The osteoporosis model was induced by ovariectomy. Serum levels of sex hormone E2、T、P、PRL、FSH、LH change in rads were observed after the rats were treated with Gushu granules and western medicine（estradiol valerate）,femoral osteoporosis pathology and biochemical indicators of change emical indicators of change were catch,too. Results Gushu granules and estradiol valerate could increase the level of E2 and T. Significant difference（P0. 005）was compared with model group. They ease osteoporosis pathological changes,elevate calcitonin,reduce bone alkaline phosphatase、tumor necrosis factor 、tartaric acid alkaline phosphatase and urine hydroxyproline（P〈 0.01 or P〈 0.05）. Conclusion Gushu granules could treatment and delay osteoporosisimprove. There was no significant difference with estrogen.