摘要
目的探讨沙棘黄酮(TFH)对动脉粥样硬化大鼠血管自噬相关基因beclin-1及LC3表达的影响,以阐明其保护粥样硬化的分子机制。方法健康清洁级雄性SD大鼠60只随机分为:正常对照组,模型对照组,沙棘黄酮低、中、高剂量组,白藜芦醇组;采用高脂饲料与维生素D3复制大鼠动脉粥样硬化模型,提取血清后采用比色法测定氧化应激指标变化,取主动脉HE染色观察病理形态学变化;ELISA技术检测血管中beclin-1蛋白及LC3蛋白表达,观察沙棘黄酮和白藜芦醇对beclin-1及LC3表达的影响。结果动脉粥样硬化时大鼠SOD、GSH-Px、CAT活性均明显下降,MDA含量明显升高;沙棘黄酮和白藜芦醇干预能使大鼠SOD、GSH-Px、CAT升高,MDA含量降低;粥样硬化时大鼠自噬相关基因beclin-1及LC3表达降低,沙棘黄酮和白藜芦醇干预能够上调beclin-1及LC3蛋白的表达。结论沙棘黄酮可明显上调粥样硬化大鼠自噬基因beclin-1蛋白和LC3蛋白的含量,引起机体自噬抑制动脉粥样硬化的发生和发展,发挥抗粥样硬化作用。
Objective To explore the effects of total flavones of TFH on expression of beclin-1 and LC3 in atherosclerosis rats. And to explore the molecular mechanism of TFH protecting atherosclerosis. Methods Atherosclerosis rats model was established by feeding the animals with a high lipid diet. Take the rat serum for the determination of related parameters of oxidative stress changes,The aorta were taken for pathological sections. The expression of beclin-1 in aorta of Atherosclerosis rats which were affected by TFH were analyzed by Elisa. Results Atherosclerosis rats SOD,Gsh-px and CAT activity were significantly decreased,MDA content increased significantly;TFH and Resveratrol intervention can make SOD,GSH-px and CAT in rats,reduce MDA content;Both Resveratrol and TFH could increase markedly the expression of beclin-1 and LC3 protein in atherosclerosis rats.Conclusion TFH can up-regulate significantly the expression of beclin-1 and LC3 in atherosclerosis rats.
出处
《云南中医学院学报》
2014年第4期13-16,共4页
Journal of Yunnan University of Traditional Chinese Medicine
