不同证型复感儿与免疫指标的相关性研究

Study on the Relationship between Different TCM Syndrome Types of RRTI and Immune Indexes

目的探讨非急性感染期肺脾气虚证、脾虚肝旺证复感儿与免疫球蛋白(IgA、IgG、IgM)和T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)等免疫指标的相关性,为不同证型的复感儿提供微观辨证指标,以促进中医辨证理论的现代化研究。方法检测150例肺脾气虚证和150例脾虚肝旺证复感儿非急性感染期血清IgA、IgG、IgM及CD3+、CD4+、CD8+、CD4+/CD8+等水平,另选220例健康儿童作为对照分析。结果 1与健康儿童对照组相比较,肺脾气虚证和脾虚肝旺证复感儿IgA和IgG水平均降低(P<0.05),CD4+细胞百分率降低(P<0.05),CD8+细胞百分率增高(P<0.05),CD4+/CD8+比值降低,且具有统计学意义(P<0.01),其余指标均无统计学意义(P>0.05);2两中医证型组组间相比较,免疫球蛋白和T淋巴细胞亚群等微观指标均无统计学意义(P>0.05)。结论肺脾气虚证、脾虚肝旺证复感儿非急性感染期体液免疫功能低下,细胞免疫功能紊乱,肺脾气虚证、脾虚肝旺证复感儿与免疫球蛋白、T淋巴细胞亚群等免疫指标间存在一定关联,但不具备正负相关性。

Objective To study the relationship of immunoglobulins（IgA,IgG,IgM）、T cell subsets（CD3＋,CD4＋,CD8＋,CD4＋/CD8＋）and children with recurrent respiratory infection in the Lung-spleen-qi-deficiency,spleen-deficiency-hyperactive-liver-fire.Methods We detected 150 cases in the Lung-spleen-qi-deficiency and 150 cases in the spleen-deficiency-hyperactive-liver-fire of RRTI in no nacute infection period of serum IgA,IgG,IgM and CD3＋,CD4＋,CD8＋,CD4＋/CD8＋level. A total of 220 children were selected as health control group. Results ① Compared with healthy control group,the level of IgA,IgG and CD4＋cells in the different types of RRTI group decreased（P〈0.05）,the percentage of CD8＋cells increased（P〈0.05）,CD4＋/CD8＋ratio decreased,and there was a significant difference（P〈0.01）. No significant differences were foud in other indexes（P〉0.05）. ②Compared with the two Chinese medicinesyndromes,the level of IgA,IgG,IgM,CD3＋,CD4＋,CD8＋and CD4＋/CD8＋showed no significant difference（P〉0.05）.Conclusion In children of recurrent respiratory tract infection（non-acute infection period）,the syndrome type of traditional Chinese medicine and immune globulin,T cell subsets in microcosmic indexes have a certain correlation,but no significant positive or negative correlation.