不同训练负荷大鼠骨骼肌肌浆网钙转运能力的变化

EFFECT OF DIFFERENT EXERCISE INTENSITY TRAINING ON CALCIUM TRANSPORT IN RAT SKELETAL MUSCLE SARCOPLASMIC RETICULUM

目的观察不同训练负荷条件下大鼠骨骼肌肌浆网钙转运能力的变化,为武警官兵科学训练提供实验依据。方法 24只SD大鼠随机分为正常对照组（n=8）,在笼内自由活动;有氧运动组（n=8）,在跑台上每天定时进行递增速度运动,运动强度为64%～76%VO2max;无氧运动组（n=8）,在跑台上每天定时进行高速间歇运动,强度〉80%VO2max。上述各组4周后处死取样,采用高速离心机提取骨骼肌肌浆网,采用紫外分光光度计检测钙转运能力的变化。结果有氧运动组大鼠骨骼肌肌浆网Ca^2＋最大摄取量和最大释放量分别为（57.17±4.62）和（33.54±4.97）nmol/mgprot,均明显高于无氧运动组〔（33.18±3.90）、（16.17±2.89）nmol/mgprot,P〈0.05〕和正常对照组〔（34.67±4.25）、（24.81±3.75）nmol/mgprot,P〈0.05〕,3组间骨骼肌肌浆网Ca^2＋最大摄取速度和最大释放速度的差异也呈同上趋势（P〈0.05）。结论有氧运动更有利于提升骨骼肌肌浆网对Ca^2＋的转运功能。

Objective To observe the effect of different exercise intensity training on calcium transport in SD rat skeletal muscle sarcoplasmic reticulum,so as to provide experimental basis for military scientific exercise training.Methods Twenty-four SD rats were randomly divided into 3 groups： normal control group（n = 8,moved freely in the cage）;aerobic exercise group（n =8,treated with progressively incremental speed exercise on the treadmill,and with the exercise intensity of 64% ～ 76% VO2max）;anaerobic exercise group（n =8,treated with high-speed intermittent excercise,and 〉80% VO2max）.The skeletal muscle sarcoplasmic reticulum were isolated by ultracentrifugation to detect their calcium transport changes after 4 weeks of training. Results The maximum calcium uptake and release of skeletal muscle sarcoplasmic reticulun in aerobic exercise group 〔（57.17 ±4. 62）,（33. 54 ±4. 97）nmol /mg prot respectively〕were higher than those in anaerobic exercise group 〔（33. 18 ± 3. 90）,（16. 17 ± 2. 89）nmol /mg prot respectively〕,and normal control group〔（34.69 ±4.25）,（24.81 ±3.75）nmol /mgprot respectively〕（P〈0.05）. The changes of maximum speed of calcium uptake and release of skeletal muscle sarcoplassmic reticulun in 3 groups were similar to the above-mentioned changes. Conclusion Aerobic exercise is more conducive to enhancing skeletal muscle sarcoplasmic reticulum Ca^2＋ transport function.