微波法提取全叶马兰总黄酮成分工艺优化

Optimization technique of flavonoids from Kalimeris integrtifolia by microwave extraction method

目的:考察影响微波法辅助提取全叶马兰总黄酮的主要因素,用正交实验确定了最优提取工艺。方法:采用微波辅助法进行黄酮类化合物的提取,分析比较料液比例、乙醇浓度提取时间和微波功率对总黄酮提取量的影响,用紫外分光光度法测定黄酮含量。结果:优选了测定波长及显色条件等分析条件,测定方法的回归方程y=0.0213x+0.0051相关系数R2=0.9933。单因素实验考察了乙醇浓度、微波功率、提取时间、料液比四个因素对全叶马兰黄酮类化合物提取效率的影响。通过正交试验确定了最佳的提取工艺条件为物料比为1:10、提取时间40s、微波功率406W、乙醇浓度60%。结论:该方法与传统的提取方法相比,提取率高,省溶剂,大大提高了提取效率。

Objective：To study the main factors those affect the extraction of total flavones in Kalimeris integrtifolia by microware extraction method according to the orthogonal design in this paper.Methods：Flavonoids compounds were extracted by microwave method.The effects of extraction microwave power,alcoholconcentration,extraction time and solid-liquid mass ratio on the extraction of flavonoids of Kalimeris integrtifolia were analyzed.The content of flavonoids was determinated by ultraviolet.Results：The determination conditions of UVVis,the color reaction selection,color terms and wavelength detection were studied in this paper.The regression equation is y=0.0213x＋0.0051,r=0.9933.Four main factors including extracting solvent concentration,extraction temperature,extraction time and ratio of solvent to sample were studied.By using single factor analysis method and the orthogonal L9（3^4）analysis,the best extraction conditions were determined,that is,40%ethanol concentration,microwave power was 406W;the extraction time was 40s;solid-liquid mass ratio was 1：10.Conclusion：Compared with traditional methods of extraction,this method serves high yield and provincial solvent,which greatly improves extraction efficiency.