睾丸酮降解菌的分离鉴定及降解特性

Isolation and Characterisation of a Testosterone-Degrading Bacterium

为筛选获得具有降解环境雄激素的菌株，从福建莆田的土壤中分离得到1株能够高效降解睾丸酮的菌株，命名为C53-20。该菌经过常规的形态和16SrDNA序列同源性分析，鉴定为克雷伯氏肺炎菌（Klebsiella pneumonia）。该菌在35～40℃、pH6.0～8.0具有较高的降解活性，其最适值分别为温度37℃和pH6.5。该菌能够以睾丸酮为唯一碳源生长，12h内对1mmol·L-1睾丸酮的降解率为45％。该菌的筛选获得为进一步研究与睾丸酮降解相关的微生物基因提供资源。

One bacterium ,which has testosterone degradation efficiently ,was isolated from the sludge ,Fujian and named C53-20. The results of morphological characteristics and the similarity analysis of the 16S rDNA sequence suggest that it is a Klebsiella pneumonia .In addition ,testosterone degradation activity in 35 -40℃ and pH6.0-8.0 range was assayed ,and our results suggest that the optimum activity is at 37℃ and pH6.5 ,respectively .Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45% ,with 1mmol·L-1 concentrations within 12 hours .Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.