兔脊髓缺血再灌注损伤实验的护理

Nursing of Rabbit with Spinal Cord Ischemia and Reperfusion Injury Experiment

目的探讨新西兰兔动物模型在脊髓缺血再灌注损伤建立过程的护理配合,以保证手术的顺利进行。方法新西兰大白兔18只,随机分成三组,每组6只,分为缺血再灌注组、乌司他丁后处理组及假手术组。所有参与实验的新西兰大白兔术前、术中和术后均实施严格的整体护理。结果 18只实验动物均顺利建立脊髓缺血再灌注损伤模型,术后48 h均存活,顺利完成实验。结论在脊髓缺血再灌注损伤手术中实施严格的护理措施有利于动物手术的顺利实施。

Objective To study the nursing cooperation during the establishment of spinal cord ischemia and reperfusion injury model with New Zealand rabbit, so as to ensure the successful operation. Methods 18 New Zealand rabbits were randomly divided into ischemia/reperfusion group, ulinastatin postconditioning group and sham-operation group, with 6 cases in each group. All rabbits received strict holistic nursing during preoperative period, intraoperative period and postoperative period. Results All rabbits were successfully established model of spinal cord ischemia and reperfusion injury. All rabbits were alive within 48 hours after surgery, and completed the experiment successfully. Conclusions Strict nursing measures during the establishment of spinal cord ischemia and reperfusion injury model are conducive to the smooth implementation of animal surgery.