摘要
实时荧光定量PCR技术作为一种高效的微生物定量检测法,在食品工业中具有广泛的应用前景。本文综述了实时荧光定量PCR技术检测原理,及其在食源性致病菌如金黄色葡萄球菌、大肠杆菌O157∶H7、沙门氏菌和副溶血性弧菌检测中的应用。
The Real-time PCR, which is an efficient method of quantitative detection of microorganisms, has abroad application prospects in food industry. This article introduced the principle of Real-time PCR, as well as its application in detection of foodborne pathogens, such as Staphylococcus aureus, Escherichia coli O157 ：H7, Salmonella and Vibrio parahaemolyticus.
出处
《中国食物与营养》
2014年第8期13-17,共5页
Food and Nutrition in China
基金
国家自然科学基金青年项目(项目编号:30700627)
江苏省级大学生创新创业训练计划项目(项目编号:J1030830)
