夏枯草对食管癌Eca-109细胞体外侵袭和转移的影响

Effect of Prunella vulgaris on Invasion and Metastasis of Esophageal Carcinoma Eca-109 Cells in Vitro

目的:探讨夏枯草提取物对人食管癌Eca-109细胞体外侵袭和转移的影响。方法:不同浓度夏枯草作用于食管癌Eca-109细胞48h后,观察细胞形态,利用MTT法检测细胞增殖抑制率,通过Transwell小室模型检测细胞侵袭力及细胞运动能力。结果:夏枯草处理组细胞体积变小,悬浮细胞增多,食管癌Eca-109细胞增殖率、侵袭力和转移能力降低,并呈量效和时效关系。结论:夏枯草提取物可抑制食管癌Eca-109细胞体外侵袭和转移。

[Objective] To study the effect of Prunella vulgaris on invasion and metastasis of esophageal carcinoma Eca-109 cells in vitro. [Method] Esophageal carcinoma Eca-109 cells were treated respectively with various concentration of Prunella vulgaris for 48h, then observed the cells morphology. The rate of cell proliferation inhibition was detected by MTF assay, and invasion and migration were examined in Transwell chamber. [Result] The Prunella vulgaris treated cells kept round with relatively fewer pseudopods and the volume reduced. Prunella vulgaris could effectively inhibit esophageal cancer Eca-109 cells proliferation, invasion, and migration in dose-dependent and time-dependent manner. [Conclusion] Prunella vulgaris could inhibit the invasion and metastasis of esophageal carcinoma Eca-109 cells.