小口径聚乳酸-己内酯/纤维蛋白原管形支架的构建及生物相容性评价

Establishment and Biocompatibility of a Small Diameter Tubular Scaffold of Electrospun Poly(L-lactide-co-ε-caprolactone)/fibrinogen Blended Fibers

目的探索静电纺丝技术制备小口径聚乳酸-己内酯[P(LLA-CL)]/纤维蛋白原管形支架的方法,评价支架的生物相容性,探讨其作为血管组织工程材料的可行性。方法以P(LLA-CL)、纤维蛋白原为原料,制备小口径复合管形支架,观察支架的大体形态,并用扫描电镜观察三维结构;利用溶血试验、细胞毒性试验、皮下植入试验,评价支架材料的生物相容性。结果管形支架表面呈网格状三维结构,并有大小不等、互相交通的孔隙,孔径平均直径为(4.56±1.23)μm,表面纤维平均直径(318±56)nm;P(LLA-CL)/纤维蛋白原浸提液溶血率为2.87%±0.49%;细胞毒性实验示P(LLA-CL)/纤维蛋白原浸提液较阴性对照组无明显差异(P>0.05);皮下植入试验显示P(LLA-CL)/纤维蛋白原支架炎症反应轻微,材料逐渐降解。结论通过静电纺丝技术可以构建小口径P(LLA-CL)/纤维蛋白原管形支架,并具有良好的生物相容性,可作为组织工程血管的支架材料。

Objective To fabricate a small diameter tubular scaffold of P （LLA-CL）/fibrinogen by electrospinning fiber technique, to evaluate the biocompatibility of the P （LLA-CL）/fibrinogen scaffold and its feasibility for vascular tissue engineering. Methods A small diameter tubular scaffold was fabricated by co-electrospinning blend of P （LLA-CL） and Fibrinogen. Gross morphology of the scaffold was observed and the 3-dimensional structure was observed by scanning electron microscope （SEM）. Biocompatibilities of the tubular scaffold were evaluated in vivo and in vitro by acute hemolysis test, cytotoxicity test, and short-term test of subcutanous implantation. Results A randomly oriented nanofibrous structure with a well interconnected network of pores was observed. The diameter of the fiber at the outer surface was 318 ±56 nm and the average pore diameter was 4.56 ±1.23 μm; Hemolysis rate was 2.87%±0.49%; There was no significant difference of cytotoxicity between the P （LLA-CL）/fibrinogen tubular scaffold and negative control group （P〉0.05）. After the placement in rat subcutaneous pouches, the scaffolds were gradually biodegraded with little inflammatory reaction. Conclusion The P （LLA-CL）/fibrinogen tubular scaffold can be fabricated by electrospinning fiber technique. It has good biocompatibility and could be potentially used as vascular tissue engineering scaffold material.