绿原酸对小鼠不同组织巨噬细胞增殖、分泌及吞噬功能的影响

Effects of Chlorogenic Acid on Proliferation,Secretion and Phagoeytosis Functions of Different Tissue Phagocytes in Mice

为观察绿原酸(CHA)对小鼠不同组织巨噬细胞功能的影响,采用无菌操作制备小鼠腹腔、骨髓和脾脏巨噬细胞,体外培养在含有脂多糖(LPS)和绿原酸的RPMI1640培养液中,用MTT法检测细胞增殖,ELISA和Griess法分别分析细胞分泌TNF-α和NO的水平,瑞氏-吉姆萨染色法测定巨噬细胞吞噬能力。结果与对照组和LPS组相比,腹腔、脾脏和骨髓3种不同组织巨噬细胞CHA组和LPS联合CHA组A值均有所降低,即3种巨噬细胞增殖转化率均有所下降,3种不同组织巨噬细胞CHA组和CHA+LPS组NO分泌量明显增加,且具有剂量依赖性。较之LPS组,不同组织巨噬细胞CHA组TNF-α分泌量降低,而脾脏和骨髓巨噬细胞LPS联合CHA组分泌TNF-α量显著提高,腹腔巨噬细胞组不显著。CHA显著提高LPS刺激的腹腔巨噬细胞吞噬能力。此外,CHA可显著提高环磷酰胺处理小鼠脾脏和骨髓巨噬细胞存活率,但使脾脏和腹腔巨噬细胞分泌NO量显著减少。结果提示绿原酸对小鼠不同组织巨噬细胞增殖、TNF-α和NO分泌以及吞噬能力均有不同程度影响。

In order to investigate effects of chlorogenic acid （CHA）on mice macrophages of different tis-sues,mouse peritoneal,bone marrow and spleen macrophages by aseptic preparation were cultured in vitro and stimulated with lipopolysaccharide （LPS）in RPMI1640 medium containing CHA.MTT assay,ELISA and Griess methods were used to analyze cell proliferation,cell secretion levels of TNF-α and NO,and phagocytic activity of macrophages was detected by Wright-Giemsa staining.The results showed that there were significant decreases in A values and NO secretion levels of peritoneal,spleen and bone marrow mac-rophages in CHA groups and CHA＋LPS groups compared with CHA groups and CHA＋LPS groups,and there was a dose-dependent increase.Compared with LPS groups,the levels of TNF-αsecreted from mac-rophages of different tissues showed a decrease.There was a significant increase in TNF-α secretion of spleen and bone marrow macrophages in LPS＋CHA groups,and not significant in that in peritoneal mac-rophages.CHA significantly enhanced phagocytic capacity of LPS-stimulated peritoneal macrophages.In addition,CHA significantly improved the survival of spleen and bone marrow macrophages from mouse treated with cyclophosphamide （CY）,and there was a significant reduction in the amount of NO secreted from spleen and peritoneal macrophages.The results indicated that there were some degree effects of CHA on different tissue macrophage proliferation,TNF-α and NO secretion,and phagocytic activity of the mouse.