白介素13基因多态性与哮喘发病易感性的关系

Correlation between IL-13 gene polymorphism and susceptibility to asthma

目的探讨白介素13(IL-13)基因内含子区+1923C/T多态性与哮喘发病易感性的关系。方法采用聚合酶链反应—限制性片段长度多态性技术对150例哮喘患者(哮喘组)和150例健康对照者(对照组)IL-13基因内含子区+1923C/T单核苷酸多态性进行检测,比较其基因型和等位基因分布频率。结果对照组IL-13基因内含子区+1923C/T基因型CC、CT和TT的分布频率分别为41.33%(62/150)、44.00%(66/150)和14.67%(22/150),在哮喘组分别为21.33%(32/150)、41.33%(62/150)和37.34%(56/150),两组各基因型分布频率比较差异有统计学意义(χ2=24.52,P<0.01)。CT、TT基因型者患哮喘的危险性高于CC基因型者(χ2=27.38,P<0.01)。结论 IL-13基因内含子区+1923C/T多态性是影响哮喘发病的重要候选基因,T等位基因与哮喘易感性相关。

Objective To investigate the correlation between the single nucleotide polymorphism （ SNP） of ＋1923 C/T in intron region of interleukin-13 （IL-13） gene and the susceptibility to asthma .Methods The genotypes and the allele frequency distributions of ＋1923 C/T SNP of IL-13 in 150 asthmatic patients and 150 healthy controls were detected by u-sing polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP） methods.Results The genotype frequencies of CC, CT and TT in ＋1923C/T sites of IL-13 gene were 41.33% （62/150）, 44.00% （66/150） and 14.67%（22/150） respectively in the controls, and 21.33%（32/150）, 41.33%（62/150） and 37.34%（56/150） in the asthmatic subjects .The distribution of genotype in ＋1923 C/T sites of IL-13 gene between the two groups was significantly different （χ^2 =24.52, P〈0.01）.The CT and TT genotypes carried higher risks for asthma than CC genotype （χ^2 =27.39, P〈0.01）.Conclusion The ＋1923 sites polymorphism of IL-13 gene may be an important candidate gene for asthma and the T allele in the gene might be associated with the susceptibility to asthma .