HPLC-DAD法同时测定清肺抑火片中4个成分的含量

Simultaneous Determination of 4 Components in Qingfeiyihuo Talets by HPLC- DAD

目的 建立HPLC法同时测定清肺抑火片中栀子苷、黄芩苷、大黄素和大黄酚的含量.方法 采用Waters C18色谱柱（4.6 mm ×250 mm,5 μm）,以乙睛（A）-0.2％磷酸（B）为流动相梯度洗脱,流速1.0 mL·min-1,采用DAD检测器在200～400nm波长处进行检测,柱温30℃.结果 调取不同波长的色谱图分别计算各成分的含量,栀子苷（237 nm）、黄芩苷（277 nm）、大黄素（254 nm）和大黄酚（254 nm）进样量分别在0.2825～5.650μg（r2=1）;0.719～14.383 μg（r2=0.9999）;0.0415～0.83 μg（r2 =0.9998）;0.0440～0.8795 μg（r2=0.9999）范围内呈现良好的线性关系.平均回收率（n=6）分别为97.93％,97.89％,97.91％,98.04％;RSD分别为0.14％,0.47％,0.45％,0.21％.结论 该方法快速简便,结果准确可靠,为清肺抑火片的全而质量控制提供科学的依据.

Objective： To establish a HPLC method for the determination of geniposide, baicalin, emodin and chrysophanol in Qing- feiyihuo Tablets. Methods： Waters C18column （4.6mm × 250mm, 5μm） was used with acetonitrile （A）, -0.2% acid （B） as the mobile phase gradient elution, flow rate 1 mL · min-1 and the DAD detector was used to detect wavelength at 200 - 400 nm, with 30℃ of column temperature. Results： The different wavelength chromatograms were used to calculate geniposide （237nm）, baicalin （277 nm） , emodin （254 nm） and chrysophanol （254 nm）. The sample size showed a good linear relationship in the range of 0.2825 and 5. 650 μg （ r2 = 1 ）, 0.719 - 14. 383μg （ r2 = 0. 9999）, 0.0415 - 0.83 μg （ r2 = 0. 9998） and 0. 0440 - 0. 8795 μg （ r2 = 0. 9999）. The average recovery rate （n =6） were 97.93%, 97.89%, 97.91%, 98.04% respectively, RSD = 0. 14%, 0.47%, 0.45%, 0.21% respectively. Conclusion： The method is simple, accurate and reliable and can provide the scientific basis for the whole quality control of Qingfeiyihuo Tablet.