20 612例妇科门诊患者人乳头状瘤病毒检测结果分析

HPV detection results of 20 612 gynecological outpatients

目的 了解人乳头瘤状病毒（human papillomavirus,HPV）在妇科门诊患者宫颈感染情况及其亚型分布,以了解本地区HPV感染现状,为宫颈癌的防治提供参考依据.方法 汇总分析广东省妇幼保健院2012年1月至2013年7月妇科门诊进行21种HPV基因亚型检测的20612例就诊患者结果,结合患者年龄用卡方检验及方差分析对检测结果进行统计分析.结果 ①HPV总感染率为27.0％（5565例）,其中高危型HPV的感染率为：22.1％（4553例）,以单一高危型HPV感染占61.3％（2791/4553例）;②感染率最高的三个亚型为：52型（5.7％）、16型（3.6％）及58型（2.7％）;③单个HPV16、31、33、58、52、56、66、6、11、53亚型在各年龄段感染率差异有统计学意义（P＜0.05）.结论 该院妇科门诊患者宫颈HPV感染以单一高危型HPV感染为主,感染最高亚型为HPV52型;20岁以下及50岁以上年龄女性感染率明显高于20～49岁之间年龄段的女性.

Objective To provide references for preventing and controlling of human papilloma virus （HPV） infection studying HPV genotypes and their distribution characters in gynecological outpatients.Methods Cervical tissue specimens from 20 612 gynecological outpatients which all had visited Guangdong Women and Children Hospital since Jan 2012 to July 2013 were examined with Flow-through Hybridization and gene chip technique （cape medical system）to determine the 21 HPV genotypes.SPSS 17.0 software was used to establish database and analyze the data.Chi-square test and variance components method were used in the comparison of the HPV infection rates between different age groups.Results ① Among the 20 612 specimens,HPV was detected in 5565 specimens with a positive rate of 27.0% （5565/20612）; the high-risk HPV infection rate was 22.1% （4553/20612）,the low-risk HPV infection rate 22.1% （4553/20612）,and the single and high-risk HPV infection rate 61.3% （2791/4553）.② Of all the subtypes in this study,HPV 52 had the highest infection rate （5.7%） ; HPV 16 （3.6%） came next; and HPV 58 （2.7%） took the third place.③ Between different age groups,there were statistical differences （P 〈 0.05） in the single infection rates of HPV 16,31,33,58,52,56,66,6,11,and 53.Conclusion The single and high-risk HPV infection rate at our hospital gynecological clinic was the main.HPV52 was the highest infection subtypes.The infection rate is higher in patients younger than 20 or older than 50 than in 20-49 years old patients.