亚临界水提取协同大孔树脂纯化杨树芽总黄酮

Extraction and Purification of Flavonoids from Poplar Buds with Subcritical Water Extraction Coordinated with Macroporous Resin Adsorption

采用亚临界水提取-大孔树脂纯化联用技术对杨树芽中总黄酮进行提取、纯化,以提取温度、液料比和提取时间为考察因素,以总黄酮的得率为考察指标,采用正交试验优化亚临界水对杨树芽总黄酮的提取工艺;从4种大孔吸附树脂中筛选出对总黄酮有最佳分离纯化效果的一种树脂,研究其对总黄酮静态和动态吸咐以及解吸效果.结果表明,5 MPa下亚临界水提取杨树芽总黄酮的最佳工艺条件为：提取温度180℃、提取时间10 min、液料比30∶ 1（mL∶g）,总黄酮的粗品提取得率为11.83％,纯度为13.16％.通过静态吸附试验筛选出最有效的HP-20树脂分离纯化杨树芽总黄酮,通过动态吸附试验确定应用HP-20树脂吸附分离杨树芽总黄酮的最佳工艺条件为：上样质量浓度为4 g/L,流速为60 mL/h,pH值为2～3,此时吸附量为42.69 g/L洗脱剂乙醇的体积分数为90％,解吸率为93.91％,经纯化后总黄酮的纯度为49.28％.

Subcritical water extraction coupled with macroporous adsorption resin technology was applied to extract and purify flavonoids from poplar buds. Based on the yield of flavonoids, the factors of extraction technology including extraction temperature, liquid-feed ratio was optimized through orthogonal tests. By comparison of four macroporous resins in the adsorption of flavonoids, the optimal macroporous of resin was selected, then adsorption and desorption effects were investigated. The results showed that the optimum process of subcritical water extraction were extraction temperature 180 ℃, liquid-feed ratio 30 ： l, extraction time 10 rain. The yield of crude flavonoids was 11.83 % and the purity of the product is 13. 16%. The HP-20 macroporous adsorption resin was selected to separate and purify flavonoids from poplar buds. The optimal technological conditions were solution concentration 4 g/L, flow rate 60 mL/h, solution pH 2 - 3, and eluting solvent 90 % ethanol. The purity of flavonoids can reach 49.28 %.