濒危药材红芽大戟组培快繁影响因素研究

Influencing Factors on Rapid Propagation of Knoxia corymbosa Willd

【目的】研究濒危药材红芽大戟(Knoxia corym bosa Willd)最适宜的组培快繁方法,为规模化生产红芽大戟提供依据。【方法】选取红芽大戟不同组织器官为外植体,采用不同配方的培养基培养,研究影响外植体诱导分化、增殖和生根的因素,筛选获得最佳培养基。【结果】外植体采用茎尖优于叶轴、叶片,茎尖接种于MS+1.5mg/L 6-BA+0.15mg/L NAA,萌芽率为100%,MS+3.0mg/L 6-BA+0.30mg/L NAA最适用于继代增殖培养,1/2MS+2.0mg/L IBA+0.2mg/L NAA最合适生根培养。【结论】选取合适的外植体,采用适宜的培养条件和培养基,可以有效地进行红芽大戟的组培快繁。

Objective]To establish tissue culture and rapid propagation of Knoxia corymbosa Willd,and provide advices for industrial production of Knoxia corymbosa Willd.[Methods]Different tissue or organs of Knoxia corymbosa Willd as explants are cultured in different growth media for the selection of the optimum experimental scheme.The factors affecting explants induction,differentiation,proliferation and rooting culture medium are studied for screening the best culture medium.[Results]Using meristems of young plants as explants cultured in MS+6?BA 1.5mg/L+NAA 0.15mg/L medium is the best way for inducing meristems and germination rate is 100%.MS+6?BA 3.0mg/L+NAA 0.3mg/L is suitable for the subculture value?added culture,1/2MS+IBA 2.0mg/L+NAA 0.2mg/L is suitable for rooting culture.[Conclusion]Given the appropriate culture conditions and the suitable me-dium can successfully achieve the tissue culture of Knoxia valerianoides.