关于北京地区新生儿网织红细胞百分比参考区间的探讨

Study on the reference range of reticulocyte multi-parameter in newborn from Beijing

目的 探讨北京地区新生儿网织红细胞（RET）百分比的参考区间及RET与新生儿日龄的关系.方法 选取北京同仁医院2007年1月至2012年12月期间出生的新生儿2 759名,其中男1 404名,女1 355名,平均日龄4.31d.用Sysmex XE-2100血液分析仪检测网织红细胞百分比,仪器使用前对仪器进行校准.RET变量做平方根反正弦变换后,统计结果以（x）±s表示,以双侧（x）±1.96 s作为网织红细胞参考区间.按照新生儿日龄分为9组,采用独立样本资料t检验比较不同性别新生儿间RET差异;采用单因素方差分析比较不同日龄新生儿组间RET差异,两两比较采用Bonferroni调整法.结果 男、女新生儿RET的参考区间分别为2.408％ ～ 3.438％、2.740％～3.912％.男性新生儿RET低于女性新生儿,差异有统计学意义（t=-2.564,P＜0.05）.不同日龄新生儿RET差异有统计学意义（F=138.105,P＜0.01）.同日龄男、女RET差异比较,第5天组男、女新生儿网织红细胞分别为（2.656±0.067）％、（3.295±0.149）％,差异有统计学意义（t=-2.219,P＜0.05）.新生儿RET随日龄变化而变化,有明显的峰值.结论 正常新生儿RET男女间有差异,随着日龄变化而变化.针对不同性别及日龄的新生儿网织红细胞参考区间的确定可为临床疾病诊断、治疗提供参考依据.

Objective To explore the RET reference values of newborns in Beijing Area and the relationship between the RET and the day ages of newborns.Methods 2 759 （1 404 males and 1 355 females） healthy newborns （born in Beijing Tongren Hospital from January 2007 to December 2012 with 4.31 average day age） were selected,the RET percentage was counted with XE-2100 （adjusted before in use）,after the inverse sine transformation of variable＇s square root,Statistical results show as （x） ± s,the reference value refers to bilateral （x） ± 1.96 s.The sample was divided into nine groups according to different day ages.Independent sample t served to test and compare the RET difference between two genders newborns,single-factor analysis of variance served to compare the RET difference of the different day ages newborns＇groups,Bonferroni adjustment method served to apply the pairwise comparisons.SPSS 18 was used for statistical analysis between males and females across nine age groups.Results The normal RET reference values of male and female newborns separately were 2.408％-3.438％,2.740％-3.912％.RET of male newborns was lower than that of female newborns,the difference was statistically significant （t =-2.564,P ＜ 0.05）.The RET difference of different day ages of male and female newborns was statistically significant （F =138.105,P ＜ 0.01）.The comparison of the same day newborns proves that there is RET difference between the male,female newborns of fifth day groups,respectively （2.656 ± 0.067）％,（3.295 ± 0.149）％,there is statistically significant（t =-2.219,P ＜0.05）.Newborns＇ RET vary with day ages,and there is obvious peak value.Conclusions There is RET difference between the male and female newborns,which vary with day ages.The study on the RET reference values of newborns of different genders or different day ages contributes to the clinical disease diagnosis and treatment as reference.