CCDC67基因mRNA在甲状腺乳头状癌中的表达及临床意义

Expression of CCDC67 mRNA and its role in patients with papillary thyroid carcinoma

目的 探讨甲状腺乳头状癌（PTC）及癌旁组织中CCDC67基因转录水平的差异及其与PTC临床病理特征的关系.方法 采用逆转录聚合酶链反应（RT-PCR）方法,检测了48例PTC及其相对应的癌旁组织中CCDC67的转录情况;同时对甲状腺癌细胞系（TPC-1、SW579）CCDC67基因的转录进行了检测.结果 在TPC-1、SW579细胞系中,CCDC67mRNA表达均缺失.癌旁组织中均出现CCDC67mRNA的表达（100.0％,48/48）,而在PTC中CCDC67基因mRNA表达明显下降为39.6％（19/48） （P＜0.05）.在PTC中,Ⅰ～Ⅱ期与Ⅲ～Ⅳ期的CCDC67mRNA表达率分别为60％ （12/20）、25％ （7/28）;在有淋巴结转移的PTC中CCDC67mRNA的表达率为24.1％ （7/29）,在无淋巴结转移的PTC中CCDC67mRNA的表达率为63.2％ （12/19）;在病理分级Ⅰ级和Ⅱ级中,CCDC67mRNA的表达率分别为61.1％（11/18）和26.7％（8/30）.以上各组中差异均有统计学意义（均P＜0.05）.结论 CCDC67mRNA表达的异常可能是引起CCDC67失活的原因,并可能与PTC的发生、发展及转移有关.

Objective To explore the expression of CCDC67 mRNA between papillary thyroid carcinoma （PTC） and adjacent normal tissues.Methods Reverse transcription-polymerase chain reaction （RT-PCR） was employed to detect the expression of CCDC67 mRNA in 48 cases of papillary thyroid carcinoma and their matched adjacent non-cancerous epithelium and cell lines of thyroid carcinoma.Results There was no expression of CCDC67 mRNA in SW579 and TPC-1 cell line.All cases had the expression of CCDC67 in adjacent normal tissues while the expression of CCDC67 mRNA was down-regulated significantly,only 39.6％ （19/48） in 48 cases of thyroid carcinoma.In PTC,the expression rates of CCDC67 mRNA in stage Ⅰ-Ⅱ and Ⅲ-Ⅳ were 60％ （12/20） and 25％ （7/28） and 24.1％ （7/29） and 63.2％ （12/19） in the lymph node metastasis and non-metastasis groups respectively.Also the expression rates of CCDC67 mRNA in Grades Ⅰ and Ⅱ were 61.1％ （11/18） and 26.7％ （8/30） respectively.And there was significant difference between two groups （P ＜ 0.05）.Conclusions The down-regulated expression of CCDC67 is one of reasons for inactivation of PTC.And it may play an important role in carcinogenesis and progression of PTC.