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多重PCR检测猪链球菌种及主要致病血清型 被引量：4

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摘要 根据猪链球菌种特异性基因gdh及血清2、7、9型的特异性基因cps2J、cps7H、cps9H序列,分别设计4对引物,通过对单项PCR、多重PCR扩增条件和反应体系的优化,建立了快速检测猪链球菌种及主要致病血清型2、7、9型的四重PCR方法;并利用建立的多重PCR方法对鲁西区域致病性猪链球菌的流行情况进行了调查研究。
作者 王海丽 徐公义 赵德明
机构地区 聊城职业技术学院/聊城市畜禽疫病诊疗工程技术研究中心 中国农业大学
出处 《江苏农业科学》 北大核心 2014年第8期212-213,221,共3页 Jiangsu Agricultural Sciences
基金 山东省聊城市科技攻关计划(编号:2011GNS06) 聊城职业技术学院重点项目(编号:2011LZYK06)
关键词 猪链球菌 菌种 致病血清型 多重PCR 检测方法
分类号 S852.61 [农业科学—基础兽医学]
  • 相关文献

参考文献11

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二级参考文献6

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  • 3Smith H E, Veenbergen V, Veldej V D, etal. The cps genes of Streptococcus suis serotype 1,2 and 9 : Development of rapid serotype 2 specific PCR assays[J]. Clin Microbiol, 1999, 37 (10): 3146-3152.
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共引文献4

同被引文献48

  • 1刘正飞,蔡旭旺,陈焕春,吴斌,何启盖.传染性副猪嗜血杆菌的研究进展[J].养殖与饲料,2004,3(10):7-10. 被引量：7
  • 2马增军,芮萍,杨宗泽,徐林霞,王立新.副猪嗜血杆菌主要生物学特性测定[J].黑龙江畜牧兽医,2005(1):41-42. 被引量：12
  • 3邬捷,姜永康,曹国文,肖弛.四川猪嗜血杆菌胸膜肺炎血清学调查[J].动物检疫,1990(6):21-22. 被引量：9
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引证文献4

二级引证文献11

江苏农业科学
2014年 第8期

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