不稳定型心绞痛患者CD4^+T淋巴细胞微小核糖核酸155水平与冠状动脉病变的相关性

Severity of coronary artery lesions with the expression of miRNA-155 in CD4^+T lymphocytes from unstable angina patients

目的:研究外周血CD4+T淋巴细胞微小核糖核酸-155(miRNA-155)表达与血清干扰素-γ(IFN-γ)浓度和冠状动脉(冠脉)病变严重程度的相关性。方法:经冠脉造影检查后,将64例疑似不稳定型心绞痛(UAP)患者分为UAP组(经造影确诊冠心病者32例)和对照组(经造影排除冠心病者32例)。分别于冠脉造影前18~24h采集新鲜外周血,免疫磁珠法分选出CD4+T淋巴细胞,荧光定量PCR检测miRNA-155表达,酶联免疫法检测血清IFN-γ表达。并根据造影结果对冠脉病变进行Gensini评分,分析外周血CD4+T淋巴细胞miRNA-155、血清IFN-γ水平与冠脉病变Gemini评分的相关性。结果:UAP组miRNA-155、IFN-γ水平较对照组明显升高,差异有统计学意义(均P<0.05),miRNA-155和IFN-γ与冠脉病变Gensini评分呈正相关关系(r=0.534、0.713,均P<0.05)。miRNA-155和IFN-γ呈明显正相关关系(r=0.686,P<0.05)。结论:外周血CD4+T淋巴细胞miRNA-155和血清IFN-γ水平与冠脉病变程度密切相关。

Objective： To investigate the relationship between the severity of coronary artery lesions with the expression of miRNA-155 in CD4^＋ T lymphocytes and the serum level of IFN-γ from unstable angina patients （UAP）. Method.. According to the results of coronary angiography （CAG）, 64 subjects were divided into UAP group （n=32） and normal control group （n= 32）. Circulating CD4^＋T cells were subsequently obtained prior to CAG,using a magnetic cell sorting system （MACS）. Fluorescence-based quantitative real-time polymerase chain reaction （qRT-PCR） was then used to measure levels of miRNA-155 in the isolated CD4^＋ T lymphocytes. Serum levels of IFN-γ were quantified using enzyme-linked immunosorbent assays （ELISAs）. The severity of coronary lesions by CAG was evaluated by means of Gensini coronary score system. Result ： Increased expression of miRNA- 155 in CD4^＋T lymphocytes and serum levels of IFN-γ were observed in UAP group than in normal control group （P〈0.05）. Positive correlation was found between the expression of miRNA-155 ,IFN-γ and the Gensini score of coronary artery lesion （P〈0.05）. Meanwhile, positive correlation was found between the expression of miRNA- 155 and IFN-γ concentration （P〈0.05）. Conclusion： miRNA-155 in CD4^＋ T lymphocytes and the serum level of IFN-γ are closely related to the atherosclerostic lesions of coronary arteries.