RIZ1在胶质瘤中的表达及其与预后的相关性

RIZ1 expression in gliomas and its association with patient prognosis

目的研究视网膜母细胞瘤蛋白结合锌指蛋白1（RIZ1）在不同级别脑胶质瘤组织中的蛋白表达水平以及其对患者预后的影响。方法应用免疫组织化学染色检测RIZ1和Ki-67在51例不同级别星型胶质细胞瘤中的蛋白表达情况。利用统计学方法分析RIZ1表达水平与患者临床病理学特征和预后的相关性。结果免疫组化染色显示RIZ1蛋白表达主要分布在细胞质，而Ki-67蛋白主要位于细胞核；低级别胶质瘤中RIZ1的表达[（48．83±4．34）％]高于高级别胶质瘤[（7．55±4．39）％]，差异有统计学意义（P〈0．0001）；RIZ1与Ki-67的表达负相关（r=-0．8130，P％0．0001）。Kruskal-Wa11is检验结果显示RIZ1的表达与肿瘤级别和患者年龄负相关（P〈0．05），Kap1an-Meier生存分析显示RIZ1的表达与患者无进展生存期和总生存期正相关（均P〈0．0001）；单因素分析提示RIZ1高表达和Ki-67低表达的患者预后较好，将单因素分析结果中P〈0．2的因素纳人多因素Cox回归分析发现，RIZ1的高表达是胶质瘤患者预后的保护因素（P=0．000，HR=0．164，95％CI：0．071～0．378）。结论RIZ1的表达随着胶质瘤病理级别的增高而降低，并与患者的预后明显相关，RIZ1可以作为胶质瘤治疗和判断预后的生物学依据。

Objective To investigate the expression levels of retinoblastoma protein-interacting zinc finger gene 1 （RIZ1） in gliomas of different grades and its association with patient prognosis. Methods The expression of RIZ1 and Ki-67 in 51 glioma tissues of different grades, were evaluated immunohistochemically. The correlation of RIZ1 immunoreactivity with the clinicopathological features and the prognostic value of RIZ1 in patients were analyzed. Results Immunohistochemistry results showed that RIZ1 was mainly expressed in the cytoplasm and Ki-67 was mainly located in the nuclei. We found that low grade glioma tissue had a significantly higher expression of RIZ1 compared with the high grade one （[7.55±4.39]% vs [48.83± 4.34] %, P〈0. 000 1）. There was a negative correlation between RIZ1 and Ki-67 immunoreactivity （Spearman ： r= -- 0.813 0, P〈0. 000 1）. It was also suggested that RIZ1 expression was negatively associated with tumor grade and patient age （P〈 0.05）. Kaplan-Meier survival analysis revealed a positive correlation between expression of RIZ1 and progress-free survival （PFS） and overall survival （OS） of patients（P〈0. 000 1）. Univariate analysis revealed that high RIZ1 expression and low Ki-67 expression were associated with better patient prognosis. Multivariate analysis using the Cox proportional hazards model revealed that high RIZ1 expression was significantly correlated with prognosis of patients with gliomas （P = 0. 000, HR = 0. 164,95 % CI： 0.071-0. 378）. Conclusion It is concluded that RIZ1 expression is decreased with the increase of pathological grade of gliomas, and it is associated with patients prognosis; RIZ1 may serve as a marker for treatment and prognosis prediction of gliomas.