干细胞共培养技术在医学研究中的应用

Cell Co-culture Technique in the Medical Research of Stem cells

细胞共培养技术是20世纪70年代后期发展起来的将不同种类、不同来源的细胞在同一个体系中进行培养、增殖的技术,该技术的诞生至今已经历了三十多年的时间,在共培养的细胞种类、共培养条件、共培养方法等方面均取得了很大的进展。其中,将骨髓间充质干细胞与其他种类细胞共培养,以诱导骨髓间充质干细胞定向分化的研究最为常见。该文对在神经、骨关节、心血管等系统疾病的替代治疗中有重要价值的共培养研究—骨髓间充质干细胞与不同种类的体细胞共培养作了重点介绍,以期为今后的工作提供借鉴和帮助。

Cell co-culture technique was developed in 1970s. Different kinds and different sources of cells have been cultured in the same system. Great progress have been made in the cell species, conditions, methods of cell co-culture in the past thirty years. Among them, the bone marrow mesenchymal stem cells co-cultured with the other types of cells to be induced into different kinds of cells is the most common. This paper will focus on co-culture of bone mesenchymal stromal stem cells and different kinds of somatic cells, which have clear significance in the re- placement therapy of nerve, bone, cardiovascular system disease, to provide reference and help for the future.