血清AFP、GP73和GPC3联合检测在原发性肝癌诊断中的应用

Application of the combination detection of serum AFP,GP73 and GPC3 on the diagnosis of primary hepatic carcinoma

目的探讨甲胎蛋白(AFP)、高尔基体蛋白73(GP73)、磷脂酰肌醇蛋白聚糖3(GPC3)联合检测在原发性肝癌(PHC)诊断中的临床应用价值。方法采用酶联免疫吸附试验(ELISA)检测148例PHC患者、73例肝硬化(LC)患者、57例慢性乙型肝炎(CHB)患者及85名健康人(正常对照组)血清GP73、GPC3含量,同时采用免疫化学发光法检测血清AFP含量,并对检测结果进行比较。结果 PHC组GP73、GPC3、AFP血清水平分别为346.9±208.7、143.1±66.9和(690.3±402.5)μg/L,与LC组、CHB组和正常对照组血清水平比较差异具有统计学意义(P均<0.01);血清AFP、GP73、GPC3联合检测诊断PHC的敏感性为95.9%,准确性为89.2%。结论血清AFP、GP73、GPC3联合应用能弥补单项肿瘤标志物临床应用的不足,对提高PHC的阳性检出率及鉴别诊断具有重要的临床意义。

Objective To investigate the application significance of the combination detection of alpha-fetoprotein( AFP),Golgi protein 73( GP73) and glypican 3( GPC3) on the diagnosis of primary hepatic carcinoma( PHC).Methods Enzyme-linked immunosorbent assay( ELISA) was used to detect the serum levels of GP73 and GPC3 in 148 patients with PHC,73 patients with liver cirrhosis( LC),57 patients with chronic hepatitis B( CHB) and 85 healthy subjects( healthy control group). Serum AFP was detected by chemiluminescence immunoassay. The levels of GP73 and GPC3 were compared with that of AFP. Results Serum levels of GP73,GPC3 and AFP in PHC group were 346. 9 ±208. 7,143. 1 ± 66. 9 and( 690. 3 ± 402. 5) μg /L,which were significant statistically compared with those in LC,CHB and healthy control groups( P < 0. 01). In the combination detection,the sensitivity was 95. 9%,and the accuracy was89. 2%. Conclusions The combination detection of serum AFP,GP73 and GPC3 could cover the shortage of individual detections,increase the diagnosis rate of PHC and differentiate PHC with clinical significance.