不同病理类型和级别胶质瘤细胞IDH1基因突变情况分析

Analysis of IDH1 gene mutation in different pathological types and grades of glioma cells

目的探讨国人不同病理类型和级别胶质瘤细胞中异柠檬酸脱氢酶1(IDH1)基因突变情况及其临床意义。方法应用mIDH1 R132H免疫组化学法检测87例国人不同病理类型和级别的胶质瘤标本中IDH1基因突变发生率,分析不同病理类型、不同WHO级别和不同年龄的IDH1基因突变情况。结果 87例国人胶质瘤标本中有45例(51.7%)检出IDH1基因突变,并与胶质瘤的病理类型、WHO分级以及年龄等具有相关性。结论 IDH1基因突变在WHOⅡ、Ⅲ级胶质瘤中的发生率较高,并在胶质瘤的发生、发展过程中起重要作用。

Objective To evaluate the frequency and clinical significance of isocitrate dehydrogenase 1（IDH1） gene mutation in different pathological types and different WHO grades of glioma cells in Chinese. Methods The frequency of IDH1 gene mutation was detected by mIDH1 R132 H immunohistochemistry in 87 patients with different pathological types and WHO grades of glioma specimens, and the pathological type, WHO grades and patient age of IDH1 gene mutation were analyzed. Results In the 87 glioma patients, IDH1 gene mutation was found in 45（51.7%）, which was correlated with the pathological types, WHO grades and the onset age of glioma.Conclusion The frequency of IDH1 gene mutation is high in WHO grade Ⅱ and Ⅲ gliomas. IDH1 gene mutation plays important role in the development and progression of glioma.