姜黄素对大鼠肝癌诱发初期肝脏氧化应激的影响

Effect of curcumin on hepatic oxidative stress of rats in early stage of hepatocellular carcinoma

目的:观察姜黄素对大鼠肝癌发生初期肝脏及其线粒体损伤及氧化应激的影响,探讨其在肿瘤防治中的作用。方法:通过化学法[二乙基亚硝胺(diethylnirtosamine,DEN)/四氯化碳(carbon tetrachloride,CCl4)/乙醇]诱发制备大鼠肝癌初期模型,同时以姜黄素干预6周,然后处死动物,制备肝匀浆及肝线粒体提取液,以比色法测定丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、γ-谷酰胺转肽酶(γ一GT)、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:300﹑100 mg/kg姜黄素能降低肝癌发生初期大鼠肝脏ALT、AST、γ-GT和GST活性,升高肝脏及肝线粒体SOD、CAT、GSH-Px活性和降低MDA水平。结论:姜黄素具有减轻肝癌发生初期大鼠肝细胞损伤,降低其氧化应激的作用,此作用有助于预防肝癌的发生。

Objective： To observe the effect of curcumin and its early occurrence of liver mitochondria damage and oxidative stress of rat hepatoma,and explore its role in the prevention and treatment of cancer. Methods： The early stage of hepatocellular carcinoma was established by chemical method of（ diethylnirtosamine,DEN） /（ carbon tetrachloride,CCl4） / ethanol,and the rats were administered with curcumin for six weeks. At the end of experiment,the animals were killed and the liver homogenates and liver mitochondria were extracted. Then the activities of alanine aminotransferase（ ALT）. Aspartate aminotransferase（ AST）,γ-glutamyl transpeptidase（ γ-GT）,glutathione-S-transferase（ GST）,superoxide dismutase（ SOD）,catalase（ CAT）,glutathione peroxidase（ GSH-Px）,as well as the content of malondialdehyde（ MDA）were detected by colorimetric method. Results： Curcumin（ 300,100 mg /kg） reduced the hepatic activities of ALT,AST,γ-GT and GST,increased the SOD,CAT and GSH-Px and decreased the MDA of liver and liver mitochondria of rats in early stage of hepatocellular carcinoma.Conclusions： It is suggested that curcumin could elevate hepatic injury and reduce hepatic oxidative stress of rats in early stage of hepatocellular carcinoma. This function will be helpful prevention of liver cancer.