VEGF-D在口腔鳞癌相关成纤维细胞的表达研究

The study on the expression level of VEGF-D in oral carcinoma-associated fibroblasts

目的:研究VEGF-D在口腔癌相关成纤维细胞(carcinoma-associated fibroblasts,CAFs)的表达情况以及其表达水平是否与口腔鳞癌淋巴结转移相关。方法:通过免疫组化和RT-PCR检测VEGF-D在NFs、无淋巴结转移CAFs、淋巴结转移CAFs的蛋白和mRNA表达情况。结果:成功培养和纯化CAFs,与NFs相比,CAFs阳性表达α-SMA,生长增殖速度明显增加,细胞排列混乱;VEGF-D在淋巴结转移CAFs的染色强度明显增加,用2-ΔΔCT法计算VEGF-D的mRNA表达水平分别为0.806 7±0.117、1.192 5±0.125、2.085 3±0.131。结论:与NFs相比,CAFs表达VEGF-D明显增加,且与患者淋巴结转移相关。

Objective：To explore the protein expression level of VEGF-D in oral carcinoma-associated fibroblasts and analyze its cor-relation with lymphatic metastasis of oral carcinoma. Methods：The protein and mRNA expression levels of VEGF-D in NFs,Non-meta-static CAFs and metastatic CAFs were detected by immunochemical staining and RT-PCR after digesting the primary culture human mu-cosa cells. Results：We successfully cultured and purified CAFs. The CAFs were positive byα-SMA Immunohistochemical staining,high proliferation rate,chaos cytoarchitecture and the staining intensity of VEGF-D.β-actinas used as a reference gene,the mRNA level of VEGF-D respectively showed 0. 8,1. 2 and 2. 1. Conclusion：Compared with the NFs,CAFs increased the expression of VEGF-D,and the expression levels of VEGF-D correlates with the lymphatic metastasis.