Beclin1和LC3在膀胱尿路上皮癌中的表达及意义

Expression of beclin1 and LC3 in bladder urothelial carcinoma and its clinical significance

目的探讨Beclin1和微管相关蛋白1轻链3(LC3)在膀胱尿路上皮癌中的表达及两者的相关性。方法通过免疫组化法检测56例膀胱尿路上皮癌和32例正常膀胱组织的Beclin1和LC3表达,对比表达的差异。结果膀胱尿路上皮组织Beclin1阳性表达率为66.1%(37/56),显著低于正常膀胱组织的93.8%(30/32),差异有统计学意义(P<0.01),而癌组织中LC3阳性表达率为50%(28/56),亦显著低于正常膀胱组织87.5%(28/32),差异有统计学意义(P<0.01);Beclin1和LC3表达均与患者临床分期有关(P<0.01),与患者年龄、性别及肿瘤生长方式不具有相关性(P>0.05);Beclin1和LC3在膀胱尿路上皮癌中的表达具有正相关性(P<0.01)。结论膀胱尿路上皮癌中Beclin1和LC3阳性表达率低于正常膀胱组织,两者可能均与膀胱尿路上皮癌的发生发展有关。

Objective To investigate Beclinl and LC3 in bladder urothelial carcinoma and their correlation. Meth- ods Expressions of Beclinl and LC3 in 56 specimens of bladder urothelial carcinoma and 32 specimens of normal bladder tissues were detected by immunohistochemistry methods. Results The positive expression rate of Beclinl in the specimens of bladder urothelial carcinoma （66. 1% , 37/56） was significantly lower than the rate in normal bladder tissue （93.8%, 30/32） （P 〈 0.01 ）. The positive expression rate of LC3 in the specimens of bladder urothelial carcinoma （50% ,28/56） was also significantly lower than the rate in normal bladder tissue （87.5% , 28/32） （P 〈 0. 01 ）. Beclinl and LC3 expressions were associated with clinical staging （ P 〈 0.05 ）, but not correlated with age, gender and tumor growth pattern （ P 〉 0. 05 ）. The expression of Beclinl had a positive corre- lation with LC3 in bladder urothelial carcinoma （P 〈 0.01 ）. Conclusion The expression of LC3 and Beclinl is down-regulated in bladder urothelial carcinoma tissues, which might be related to the tumorigenesis and develop- ment of bladder urothelial carcinoma.