黑腹果蝇CG18853基因编码蛋白的生物信息学分析

Bioinformatics Analysis of CG18853 Encoding Protein of Drosophila melanogaster

目的:利用生物信息学方法分析黑腹果蝇CG18853基因编码蛋白的结构和功能。方法:基于NCBI数据库中黑腹果蝇CG18853基因编码蛋白的氨基酸序列,从蛋白质的理化性质、跨膜区、信号肽、亚细胞定位、结构域、三维结构及不同物种间同源蛋白进化关系等方面进行分析。结果:果蝇CG18853蛋白的理论分子量约38.5 kDa,理论等电点为8.80。CG18853蛋白为不稳定亲水性蛋白,无跨膜区和信号肽,具有DNA光修复酶FAD结合结构域。果蝇CG18853蛋白与模板3umv.1.A有60.87%的氨基酸序列一致;CG18853蛋白与长鼻袋鼠、金鱼、拟南芥、粳稻的编码产物高度同源。结论:黑腹果蝇CG18853蛋白具有DNA光修复酶家族的典型结构,可能在细胞核中参与DNA损伤修复过程。

Objective：To analyze the structure and function of CG18853 encoding protein of Drosophila melanogaster using bioinformatics methods. Method： Based on the amino acid sequence of CG18853 encoding protein of Drosophila melanogaster from NCBI database, the bioinformatics analyses were performed, including protein physicochemical property, transmembrane region, signal peptide, subcellular lo- calization, domain, tertiary structure, the phylogenetic tree of CG18853 related proteins from different species, and so on. Result： The esti- mated theoretical molecular weight and isoelectric point of the Drosophila CG18853 protein were 38. 5 kDa and 8. 80. CG18853 protein was unstable hydrophilic protein, which contained DNA photolyase FAD -binding domain, but without transmembrane region and signal pep- tide. The amino acid sequence identity between CG18853 protein and template 3umv. 1. A was 60. 87%, CG18853 protein and its related proteins in long- nosed potoroo, goldfish, thale cress and Japanese rice showed highly homology. Conclusion： CG18853 protein contained the typical structure of DNA photolyase family and might participate in the repair of DNA damage in the nuclei of Drosophila melanogaster.