高产紫杉醇菌株发酵条件优化

Optimization of Fermentation Conditions of High- production Taxol Endophytic Fungi

目的:优化WY2菌株发酵条件,提高紫杉醇的产量,为后续研究奠定基础。方法:采用不同的培养基、初始pH、温度、摇床转速、培养时间对曼地亚红豆杉内生真菌进行组合优化培养,测定其对紫杉醇产量的影响。通过正交试验,进一步探究前体物质对紫杉醇产量的影响。结果:使用YES发酵培养基,初始pH值为6.0,培养温度为30℃,摇床转速200r/min为最佳培养条件。当向培养基中加入20mg/l的苯丙氨酸、10mg/l的乙酸铵、5mg/l的络氨酸、20mg/l的甘氨酸时,紫杉醇产量显著提高,达到547μg/l,比初始产量高337%。结论:按照优化后的培养条件,添加合适的前体物质,能够显著提高紫杉醇的产量,满足更大的医药需求。

Objective： The production of taxol endophytic fungi was improved through optimizing the fermentation conditions of WY2, which would support the foundation for following study. Method： Have explored the culture medium, the initial pH, fermentation temperature, shaker speed and the incubation time, and detected the influence on the taxus chinensis taxol production by endophytic fungi. Result： The results show that YES medium was the best culture, and the initial pH 6. 0, cultivate temperature 30℃, shaker rate 200r/min was the best fermentation conditions. The effects of some precursor on production of taxol in endophytic fungi strain WY2 also were studied. By the orthogonal experiment, it showed that when add 20 mg/l phenylalanine, 10 mg/1 of ammonium acetate, 5 mg/1 winding glycine, 20 mg/ 1 of glycine to medium, taxol production （547μg/1） was increased by 337% , compared to that of initial production. Conclusion： The op- timized culture condition and the appropriate precursor can be used to improve the production of taxol, meeting the demand of medical ap- plication.