锌指蛋白3在普通C57BL／6J小鼠组织器官中的表达差异及利拉鲁肽对其在C57BL／6J和db／db小鼠肝脏表达的影响

Zinc-finger BED domain-containing 3 expression in tissues of various organs in normal C57BL/6J mice and the changes in the ZBED3 expression levels affected by liraglutide in C57BL/6J mice and db/db mice

【摘要】目的观察小鼠各组织中锌指蛋白3（zinc—fingerBEDdomain—containing3，ZBED3）的表达差异及利拉鲁肽、葡萄糖和胰岛素刺激对C57BL／6J和db／db小鼠肝脏ZBED3表达的影响。方法实时定量PCR法比较C57BL／6J小鼠各组织中ZBED3mRNA表达差异，Western印迹检测ZBED3在C57BL／6J和db／db小鼠肝脏的蛋白表达差异及观察不同浓度利拉鲁肽、葡萄糖、胰岛素对C57BIM6J和db／db小鼠肝脏ZBED3表达的影响。结果小鼠肌肉、脾脏、肾脏、脑、脂肪、心脏、肺、肝脏均有ZBED3mRNA表达．其中肌肉表达最高。与C57BL／6J小鼠相比，db／db小鼠肝脏ZBED3蛋白表达显著升高（P〈0．01）。高浓度利拉鲁肽、葡萄糖、胰岛素均能够显著增加db／db小鼠肝脏ZBED3蛋白表达（P〈0．05）；其次，高浓度葡萄糖能明显增加c57BL／6J小鼠肝脏ZBED3表达（P〈0．01），而高浓度利拉鲁肽、胰岛素对C57BIM6J小鼠肝脏ZEBD3表达无明显影响。结论ZBED3可能作为一种新因子参与糖代谢，且利拉鲁肽、葡萄糖及胰岛素均能够调节db／db小鼠肝脏ZBED3的表达，因此，本研究推测ZBED3可能在机体糖代谢中发挥着重要的作用。

Objective To compare zinc-finger BED domain-containing 3 （ZBED3） expression in various tissues of C57BL/6J mice and the effects of liraglutide, glucose, and insulin on the levels of ZBED3 protein expression in C57BI/6J mice and db/db mice. Methods The mRNA level of ZBED3 in various tissues of C57BI/6J mice was measured by realtime PCR. The protein level of ZBED3 was measured by using western blot. Results ZBED3 mRNA levels were detected in muscle, spleen, kidney, brain, heart, lung, and liver of C57BL/6J mice, yielding the highest expression in muscle. Additionally, The liver ZBED3 levels were higher in db/db mice compared with C57BL/6J mice （ P〈0.01 ）. Furthermore, the protein expression of ZBED3 was significantly increased in liver tissues of db/db mice treated with high concentrations of liraglutide, glucose or insulin （ P〈0.05 ） , however, the expression of ZBED3 only responded to high concentration of glucose in liver tissues of C57BL/6J mice. Conclusion ZBED3 may act as a novel factor in regulating glucose metabolisrn. The expression of ZBED3 can be regulated by liraglutide, glucose, and insulin. Thus, ZBED3 may play an important role in conditioning of hyperglycemia.